This Week in Virology

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler The TWiV gang answer follow-up questions about the Ebola virus outbreak in West Africa, then discuss treatment of disseminated multiple myeloma with oncolytic measles virus. Links for this episode Use of unregistered interventions for Ebola virus disease (WHO) Response to anthrax and H5N1 incidents (CDC) Remission of disseminated cancer after treatment with oncolytic measles virus (Mayo Clin Proc) New viruses for cancer therapy (Nat Rev Micro) Cattaneo on TWiV Image credit Letters read on TWiV 298 Weekly Science Picks Alan - WTF, Evolution?! by Mara GrunbaumRich - Cold Spring Harbor Oral History CollectionKathy - Inside insidesVincent - Going Viral (Rocky Mountain Ballet Theatre) Listener Pick of the Week Kim - Nobel Prize PodcastsJohnye - The Planet is FineJennie - Counter-Zombie Dominance Plan (pdf) Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler The TWiVites present an all-ebolavirus episode, tackling virology, epidemiology, and approaches to prevention and cure that are in the pipeline. Links for this episode Risks and benefits of gain-of-function experiments (mBio) Scientists for Science US Policy for oversight of DURC (pdf) US framework for guiding H5N1 transmission expts (pdf) Extra oversight for H7N9 experiments (Science) TWiV 283: No Reston for the weary Is it Ebolavirus or Ebola virus? (virology blog) Ebola virus disease update (WHO) Ebola virus disease FAQ (WHO) Ebola hemorrhagic fever (CDC) Ebola virus disease: ecology meets economy (PLoS NTD) 2014 Ebola virus outbreak (J Gen Virol) 2014 Ebola virus outbreak (MMWR) If Ebola virus came to the US (Vox) Zaire ebolavirus in West Africa (interview with Tom Solomon) Why Ebola patients were brought to US (Wash Post) Experimental treatment and vaccines for Ebola (CDC) Aerosol transmission of Reston virus (Sci Rep) Ebola - A growing threat? (NEJM) On the cusp of Ebola vaccine (Know) Ebolavirus vaccines and antivirals (virology blog) Immune responses to Ebolavirus infection (Exp Rev Clin Imm) Antibody therapy for Ebola (Hum Vacc Immunother) Plant derived monoclonals protect macaques from Ebolavirus (PNAS) Anti-ebolavirus monoclonal cocktail (Sci Trans Med) ZMapp info sheet (pdf) ZMapp PR (pdf) High tech treatment for Ebola (ArsTechnica) ZMapp protects macaques (Sci Rep) Letters read on TWiV 297 Weekly Science Picks Alan - Historical perspective on Ebola (Tara C Smith)Rich - Tell the negative committee to shut up (Fanuel Muindi)Kathy - Art of Night (Vimeo)Vincent - Is Ebola virus going to kill me? (John Skylar) Listener Pick of the Week Peter - Dr. Michael Saag interview by Dr. Virginia CampbellDavid - PI Predictor (app, article, publication) Send your virology questions and comments (email or mp3 file) to [email protected]

Host: Vincent Racaniello Guest: Linfa Wang Vincent visits the Australian Animal Health Laboratory in Geelong, Australia and speaks with Linfa about his work on bats and bat viruses. Links for this episode Hendra virus vaccine (Emer Inf Dis) Comparison of bat genomes (Science) Endogenous retroviruses in bats (Retrovirol) The Bat Pack Bats in Geelong (YouTube) Video of this episode - view at YouTube Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Rich Condit The TWiVome discusses an miRNA based strategy to mitigate risk of gain of function studies, and identification of a second receptor required for Lassa virus entry. Links for this episode miRNA strategy to minimize risk (Nat BioT) NIH Office of Science Policy NIH recombinant DNA guidelines Second receptor for Lassa virus entry (Science) Lassa fever (Wikipedia) Fever! The discovery of Lassa virus (TWiV 9) Letters read on TWiV 294 Weekly Science Picks Alan - Nicky Bay macrophotography (Flickr)Rich - FASEB Third annual BioArt competitionVincent - Up Close podcast (vrr episode)Dickson - Science fun (YouTube) Listener Pick of the Week Mark Martin - Average bacteriophageKonrad - Scholarpedia Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Rich Condit The TWiV team reviews the discovery of old vials of smallpox virus at NIH, anthrax and influenza mishaps at CDC, the baby who was not cured of HIV, Cambridge Working Group, and sacking of NSABB members. Links for this episode Newly discovered smallpox (CDC) Infectious smallpox in FDA storage (FDA) Report on potential anthrax exposure (pdf, CDC) CDC Director on unsafe practices (NY Times) HIV returns to baby (NY Times) Original HIV baby cured article (NEJM) Cambridge Working Group NSABB members released (ScienceInsider) Letters read on TWiV 294 Weekly Science Picks Alan - OptivisorRich - Noller lab ribosome moviesVincent - Creepy dreadful wonderful parasitesDickson - National Center for Science Education Listener Pick of the Week Mark - The Machinery of Life by David GoodsellBasel - iBioEducation Send your virology questions and comments (email or mp3 file) to [email protected]

Host: Vincent Racaniello Guests: Melissa Churchill, Alex Khromykh, Gilda Tachedjian, and Paul Young Vincent visits Melbourne, Australia and speaks with Melissa, Alex, Gilda, and Paul about their work on HIV infection of the central nervous system, West Nile virus, microbicides for HIV, and the Koala retrovirus. Links for this episode Where does HIV hide? (Curr Op HIV AIDS) Subgenomic flavivirus RNA (Viruses) Vaginal lactic acid and HIV (J Antimicro Chem) Koala retroviruses (Retrovirology) Coming soon - Video of this episode at YouTube Send your virology questions and comments (email or mp3 file) to [email protected]

Host: Vincent Racaniello Guests: Wade Blair, Matt Dickson, Nicole Kallewaard-Lelay, and Ken Miller Vincent visits Medimmune and speaks with Wade, Matt, Nicole, and Ken about why they work in industry and their daily roles in a biotechnology company. Links for this episode Medimmune, LLC Medimmune on Twitter Medimmune history and products Video of this episode - view at YouTube Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Rich Condit, and Kathy Spindler Guests: Clodagh O'Shea and Ron Fouchier Vincent, Rich, and Kathy and their guests Clodagh and Ron recorded this episode at the 33rd annual meeting of the American Society for Virology at Colorado State University in Ft. Collins, Colorado. Links for this episode Viral polymer that inactivates tumor suppressors (Cell) Mutations driving airborne transmission of influenza H5N1 virus (Cell) Transmission of influenza H7N1 virus in ferrets (J Virol) Ron Fouchier on TWiV #177 Photo credit: Matt Evans Thanks to David Quammen for the title Video of this episode - view at YouTube Weekly Science Picks Rich - No sexual transmission of HCV (Am J Gastro)Vincent - Made with code (blog post)Kathy - Beautiful math images (and 50 Visions of Mathematics) Listener Pick of the Week Jon - Advances in Life Sciences winners (YouTube)Dave - Adam Ruben Send your virology questions and comments (email or mp3 file) to [email protected]

Host: Vincent Racaniello Guests: Janet Butel and Rick Lloyd Vincent meets up with Janet Butel and Rick Lloyd at Baylor College of Medicine to talk about their work on polyomaviruses and virus induced stress. Links for this episode SV40 microRNAs (PLoS Path) SV40 seroprevalence (J Infect) Viruses and stress granules (PLoS Path) Viral inhibition of stress granule formation (Cell Host Micr) Video of this episode - view below or at YouTube Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Vinny and the capsids answer listener questions about the definition of life, state vaccination laws, the basic science funding problem, viral ecology, inactivation of viruses by pressure, and much more. Links for this episode Varun's research poll Letters read on TWiV 289 Weekly Science Picks Alan - The Science of Good Cooking Vincent - The Shooter Report (pdf)Kathy - Daily overviewDickson - Girl rising Listener Pick of the Week Johnye - Ri channelRicardo - Open spin microscope (Nikon gallery, plans)Chris - The microbiome game Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The Twivsters discuss how reverse transcriptase encoded in the human genome might produce DNA copies of RNA viruses in infected cells. Links for this episode Should variola virus be destroyed? (poll at virology blog) DNA complementary to non-retroviral RNA viruses (Sci Rep) Integration of arenavirus DNA into cell genome (virology blog) Infectious respiratory syncytial DNA (PNAS) Tysabri and PML JC and PML (J Imm Res) Igor Koralnik laboratory Jean-Luc Doumont (Kathy's pick, TWiV 268) Bullet points kill (TED) Letters read on TWiV 288 Weekly Science Picks Rich - LuvalampsAlan - ExperimentVincent - American Society for Virology on FacebookKathy - LEGO female scientistsDickson - The Oldest Living Things in the World by Rachel Sussman Listener Pick of the Week Basel - A Treatise on the small-pox and measles by Abu-Bakr Al-Razi Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Kathy Spindler, and Matt Frieman Matt updates the TWiV team on MERS-coronavirus, and joins in a discussion of whether we should further regulate research on potentially pandemic pathogens. Links for this episode MERS stories at CIDRAP MERS at CDC MERS in the US (CDC) MERS-CoV map MERS-CoV infection after camel exposure (EID) MERS-CoV in dromedaries from Egypt, Sudan, Ethiopia (EID) MERS-CoV antivirals (one, two, three, four) Experiments with novel potential pathogens (PLoS Med) Unacceptable risks (Bull Atom Sci) TWiX Bingo (thanks, Mark!) Letters read on TWiV 287 Weekly Science Picks Matt - Forgive me, scientistsAlan - Do not linkVincent - bioRxivKathy - Snail photographsDickson - Solar roadways Listener Pick of the Week Daniel - Benz Biome concept car Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello and Alan Dove Guests: Julie Pfeiffer and Paul Duprex Vincent and Alan meet up with Julie and Paul at the General Meeting of the American Society for Microbiology in Boston, to talk about their work on the pathogenesis of poliovirus and measles virus. Links for this episode Threading the NEIDL (YouTube) Transmission of measles virus from macaques (J Gen Virol) Tropism of green measles virus in macaques (J Virol) Intestinal microbiota promote enteric virus replication (Science) Bacterial LPS enhances poliovirus stability (Cell Host Micr) Video of this episode - view below or at YouTube Weekly Science Picks Vincent - ASM Live 2014Alan - I will not follow the herdPaul - Invisible ThreatJulie - The importance of stupidity in biological research Listener Pick of the Week Neil - WEHI movies and VIZBI Send your virology questions and comments (email or mp3 file) to [email protected]

Host: Vincent Racaniello Guests: XJ Meng and Sarah McDonald Vincent meets up with XJ and Sarah at Virginia Tech to talk about their work on viruses of swine and rotaviruses. Links for this episode Hepatitis E virus (Int J Env Res Pub Health) Porcine and human anelloviruses (J Virol) Origin of porcine epidemic diarrhea virus in US (mBio) Distinguishing rotaviruses (J Virol) Molecular epidemiology of rotaviruses (J Virol) Core domains in rotavirus polymerase encapsidation (J Gen Virol) Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Rich Condit, and Kathy Spindler The TWiV team discusses how skin scarification promotes a nonspecific immune response, and whether remaining stocks of smallpox virus should be destroyed. Links for this episode Skin scarification protects against vaccinia virus disease (J Virol) Smallpox research agenda (PLoS Path) WHO report on variola research (pdf) Review of smallpox research program for WHO (pdf) How I record my lectures (virology blog) Letters read on TWiV 284 Weekly Science Picks Rich - EPCOTVincent - Medical BiotechnologyKathy - Alan Alda interview and Flame Challenge winnerDickson - NASA live from space Listener Pick of the Week Nicola - 50 cent microscope (be a beta tester)Jon - Camtasia Studio Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler Guest: Jens H. Kuhn Jens speaks with the TWiV team about filoviruses, including the recent Ebola virus outbreak in Guinea. Links for this episode Review of Zanamavir clinical trials (Brit Med J) Ebola virus disease in Guinea (NEJM) Filovirus nomenclature (virology blog) Ebola virus disease, West Africa (ProMedMail) How lethal are ebolaviruses? (virology blog) Letters read on TWiV 283 Weekly Science Picks Alan - AMNH digital special collectionsVincent - Viral entry into host cells (Stefan Pöhlmann PhD, Graham Simmons PhD, eds)Kathy - Papilloma song lyrics (pdf)Jens - The Logic of Chance by Eugene V. Koonin Listener Pick of the Week Joe - Poor showing of 2012 influenza vaccine (Nature)Phil - Emerging disease or emerging diagnosis? (YouTube) Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler The TWiV team reviews a meta-analysis of clinical trial reports on using Tamiflu for influenza, and suggestions on how to rescue US biomedical research from its systemic flaws. Links for this episode World Malaria Day Review of oseltamivir clinical trials (Brit Med J) Effectiveness of Tamiflu questioned (NHS choices) Tamiflu campaign (Brit Med J) Rescuing US biomedical research (PNAS) Letters read on TWiV 282 Weekly Science Picks Alan - Northampton AirportVincent - Particle FeverKathy - Turn iPhone into microscopeRich - My favorite science gifs Listener Pick of the Week Nathan - The Ascent of Man (YouTube) Send your virology questions and comments (email or mp3 file) to [email protected]

Host: Vincent Racaniello Guest: Peter L. Salk Vincent meets up with Peter L. Salk to talk about development of the first poliovaccine, eradication of poliomyelitis, and Jonas Salk's 100th birth anniversary. Links for this episode Jonas Salk Legacy Foundation The Cutter Incident by Paul Offit Splendid Solution by Jeffrey Kluger (review) Coming soon: Video of this episode Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiVmeisters answer listener email about the NEIDL, negative results, patenting MERS-coronavirus, human papillomavirus transmission, canine distemper virus, and much, much more. Links for this episode Poxvirus viability in historical relics (EID) TWiM 74: It came from the Siberian permafrost How to read a scientific paper Image credit: Not only quilts Letters read on TWiV 280 Weekly Science Picks Alan - Heartbleed explanationVincent - Agriculture science today podcastKathy - Quizlet flashcards (online and iTunes)Rich - Why nothing is truly alive Listener Pick of the Week Lance - Myles Power (YouTube and Facebook)Raihan - Cheap DIY microscopeGerald - DaVinci PressNeal - I'm a virus Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler Vincent, Alan, and Kathy reveal how a retrovirus in the human genome keeps embryonic stem cells in a pluripotent state, from where they can differentiate into all cells of the body. Links for this episode Retrovirus needed for stem cell identity (Nat Struc Mol Biol) HERV-H is a marker for pluripotency (Retrovirology) Letters read on TWiV 279 Weekly Science Picks Alan - HeathkitVincent - How to think about autism risk and Autism prevalenceKathy - Distortions Listener Pick of the Week Joseph - The real process of scienceMarion - ZometoolKevin - Audioimmunity and Emmunity Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Vincent, Dickson, Alan, and Kathy discuss disruption of the ccr5 gene in lymphocytes of patients infected with HIV-1. Links for this episode Gene editing of ccr5 in AIDS patients (NEJM) HIV gets the zinc finger (TWiV 144) Genome engineering with zinc finger nucleases (Genetics) Photo credit: Watty's Wall Stuff Mice lie, monkeys exaggerate t-shirt design (thanks, Christophe) Letters read on TWiV 278 Weekly Science Picks Alan - Digital scale model of solar systemDickson - Font of knowledgeVincent - Measles outbreaks trends, and NYC measles (one, two)Kathy - Winner, funding basic science to revolutionize medicine Listener Pick of the Week Stephen & Jon - Watty's Wall StuffJohyne - Macro views of snowflakesRicardo & Stephen - Vaccine exemptionsBill - Books by John JanovyMarshall - Animation of DNA replicationSteve - Debunking influenza vaccine myths Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Glenn Rall, Ann Skalka, and Vincent Racaniello Glenn and Ann meet up with Vincent to talk about his career in science and science communication. Video of this episode - view at YouTube Send your virology questions and comments (email or mp3 file) to [email protected]

Host: Vincent Racaniello Guests: Susan Baker and Thomas Gallagher Vincent meets up with Susan Baker and Tom Gallagher at Loyola University to talk about their work on coronaviruses. Links for this episode Papain-like viral proteases (Virus Res) MERS-CoV papain-like protease (Virology) Mouse model for MERS-CoV (PNAS) Receptor and MERS-CoV infection (J Virol) Letters read on TWiV 276 Video of this episode - view at YouTube Weekly Science Picks Vincent - First demonstration of Macintosh, 1984 Listener Pick of the Week Lance - The Signal and the Noise by Nate Silver Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello and Rich Condit Guest: Eugene Koonin Vincent and Rich meet up with Eugene Koonin to talk about the central role of viruses in the evolution of all life. Links for this episode Virocentric view of evolution (Curr Op Virol) Proposed order Megavirales (Arch Virol) Pithovirus (PNAS) Selfish virus-like elements (Virol J) Pandoraviruses are derived Phycodnaviruses (Biol Direct) Video of this episode - view below at YouTube Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiV team discusses recent cases of polio-like paralysis in California, and the virome of 14th century paleofeces. Links for this episode MERS-CoV in dromedary camels (EID) Virologist Al Kapikian dies Polio-like illness in CA (ProMedMail) Acute flaccid paralysis AFP in India (EID) Enterovirus 68 (MMWR) EV 68 CNS infection (Arch Path Lab Med) Polio-free does not mean paralysis-free (The Hindu) Viruses in 14th century coprolite (AEM) Coprolite and paleofeces Ancient poxvirus (EID) Giant coprolite (WordADay) Letters read on TWiV 274 Weekly Science Picks Alan - The science of science communicationRich - Command and Control by Eric SchlosserDickson - Top 10 plant virusesVincent - Is the Nobel Prize good for science?Kathy - Time to rethink graduate and postdoc education (YouTube) Listener Pick of the Week Jon - Lessons from a Kansas graveyardMike - Cross a fashion designer with a microbiologist Send your virology questions and comments (email or mp3 file) to [email protected]

Host: Vincent Racaniello Guests: W. Ian Lipkin and Thomas Briese Vincent meets up with Ian and Thomas to discuss their finding that MERS-coronavirus has been circulating in dromedary camels in the Kingdom of Saudi Arabia since at least 1992. Links for this episode MERS-CoV in dromedary camels (mBio) TWiV 251: Don't kiss the camel TWiV 247: Today's weather in virology TWiV 239: Filterable camels Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Rich Condit, and Kathy Spindler The TWiVome dissect the finding that interferon lambda alleles predict the outcome of hepatitis C virus infection. Links for this episode Interferon lambda and HCV outcome (Cell Host Micr) Laser capture microdissection (Wikipedia) HCV dependent fluorescent reporter (Nat Biotech) Bats and viruses (PLoS Path) White nose syndrome (PLoS Path) Mouse phenome database Letters read on TWiV 273 Weekly Science Picks Rich - Think like a scientist (one, two); Science controversiesDickson - Visualization challenge 2013Vincent - Orphan BlackKathy - Alberta worm invasion project Listener Pick of the Week Timothy - How do vaccines cause autism?Anne - Luke Jerram's glass virusesJim - NLM Communications Engineering BranchSteve - Synapse by synapseJacob - Tiny technology creates a buzz Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiV team describes aphid control by using a viral capsid protein to deliver a spider toxin to plants, and a human endogenous retrovirus that enhances expression of a neuronal gene. Links for this episode Toxin delivery to plants by viral capsid protein (Nat Biotech) Pea enation mosaic virus (ViralZone) Image credit: Aphid membrane feeding chamber (Sci Rep) Aphid feeding chamber (YouTube) Parafilm (Wikipedia) Human endogenous retrovirus enhances neural gene (PNAS) Flu, common cold, alternative medicine (NCCAM) Cold vs flu (CDC) Phage hunters Bed bugs and infectious disease (PLoS Path) Letters read on TWiV 272 Weekly Science Picks Alan - Statistical error (Nature)Rich - 12 oldest animal speciesDickson - When liquids collideVincent - HIV denial and just asking questionsKathy - Sun's canyon of fire Listener Pick of the Week Sandra - Women physicists keep female students psychedJudi - HHMI's BiointeractiveJacob - Influenza virus HA Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Ashlee Bennett The TWiV crew discusses two reports on viruses that might have crossed kingdoms, from plants to honeybees and from plants to vertebrates. Links for this episode Tobacco ringspot virus in honeybees (mBio) Plant virus that switched to vertebrates (PNAS) CDC influenza activity and surveillance With Esmeralda at ASM Biodefense (jpg) Image credit: Virginia Tech Learning Resources Center Letters read on TWiV 271 Weekly Science Picks Alan - Nagasaki bombing (YouTube)Rich - GoPro Red Bull Stratos (YouTube)Dickson - Solar orbsVincent - Socrative Listener Pick of the Week Basel - Pathology course onlineRussell - Sochi 2014: Encyclopedia of spendingYegor - Visual Science Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello and Rich Condit Guests: Dennis Hruby and Yoshihiro Kawaoka Vincent and Rich discuss avian influenza virus and an antiviral drug against smallpox with Dennis and Yoshi at the ASM Biodefense and Emerging Diseases Research Meeting in Washington, DC. Links for this episode ASM Biodefense Meeting Influenza H5N1 transmission (Virus Res) Aerosol transmission of H5N1 virus in ferrets (Nature) Characterization of H7N9 virus from humans (Nature) ST-246 efficacy in primates (AAC) Antiviral options for biodefense (Curr Op Virol) ST-246 safety in humans (Antimicrob Agents Chemother) Gain-of-function experiments (Science) Letters read on TWiV 270 Video of this episode - view at Vimeo Weekly Science Picks Vincent - Quanta MagazineRich - Colour is in the eye of the beholder Listener Pick of the Week Kehau - Beautiful but deadly viruses Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The complete TWiV team reviews evidence for sensing of herpesviral DNA in the nucleus by the cell protein IFI16. Links for this episode IFI16 is a nuclear pathogen sensor (Cell Host Micr) Nuclear IFI16 sensing of and antagonism by herpesvirus (PNAS) Acetylation regulates IFI16 location (PNAS) Letters read on TWiV 269 Weekly Science Picks Kathy - Female conveners and speakersAlan - SandgrainsRich - 2013 Lamborghini AventadorVincent - Happy Birthday Mac and 128K Mac teardownDickson - Farmdominion Listener Pick of the Week Peter - The Microscope and The Next Global KillerRichard - Glass viruses Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Kathy Spindler, and Ashlee Bennett Vincent, Alan, Kathy, and Ashlee discuss fomites in physicians offices, plant virus factories involved in aphid transmission, and clues from the bat genome about flight and immunity. Links for this episode How viruses cause disease (Coursera) How clean is your iPad (Which?) Meeting announcement: From emerging to pandemic viruses CaMV virus factories involved in aphid transmission (J Virol) Caulimovirus (ViralZone) CaMV responds instantly to vector (eLIFE) Aphid acquiring CaMV (J Virol) Bat genomes, flight, and immunity (Science) Letters read on TWiV 268 Weekly Science Picks Kathy - Jean-Luc Doumont (slides, communicating science, website, Scitable)Alan - Wireless thermometerVincent - LORiOLA viral necklaces Listener Pick of the Week Jessica - Knit icosahedronRobert - Practical computing for biologists by Haddock and DunnStephen - Fourteen years of US weather (YouTube) Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler Vincent, Alan, Rich and Kathy review a protease essential for influenza pathogenesis in mice, and directionality of rhinovirus RNA exit from the capsid. Links for this episode Protease essential for influenza pathogenesis in mice (PLoS Path) Rhinovirus uncoating is directional (PLoS Path) Fluorescence correlation spectroscopy Capillary electrophoresis (Wiki) Letters read on TWiV 267 Weekly Science Picks Kathy - Amazing mapsAlan - Florida to NJ in 156 seconds (YouTube)Rich - Whiteout over Great Lakes from SpaceVincent - LORiOLA viral necklaces Listener Pick of the Week Carol - Knit picornavirusRobert - RNA: Life's Indispensible Molecule by James Darnell,and Biochemical Pathways by Michal and Schomburg Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler Vincent, Alan, and Kathy discuss finding viruses in outer space, varying results obtained from personal genetic testing, and depletion of CD4 cells during HIV infection by pyroptosis. Links for this episode We should hunt for viruses in space (New Scientist) I had my DNA picture taken (NY Times) CD4 cell death by pyroptosis (Nature) Abortive HIV infection in lymphoid tissue (Cell) IFI16 sensor required for HIV mediated cell death (Science) PubMed Commons Preferential CTL targeting of Gag (Cell Rep) Exonic transcription protein binding (Science) Letters read on TWiV 266 Video of this episode - view at YouTube Weekly Science Picks Kathy - 17 mundane things, mind blowing viewsAlan - SnowovelVincent - Public's views on human evolution Listener Pick of the Week Emily - Dengue pillow Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler In their final episode of the year, the TWiV team reviews ten compelling virology stories from 2013. Links for this episode Antiviral RNAi (TWiV 255, 256, 263) MERS-CoV (TWiV 215, 223, 224, 233, 239, 247, 251, 258) Placental trophoblasts confer viral resistance (TWiV 241) Origin of REV (TWiV 248) Phage T7 gets a cat scan (TWiV 220) It's a cGAS! (TWiV 222) Dual virus-host arms race (TWiV 242) Pandoraviruses (TWiV 246, 261) CMV based SIV vaccine (TWiV 254) Threading the NEIDL Letters read on TWiV 265 Video of this episode - view at YouTube Weekly Science Picks Kathy - Women in ScienceAlan - ContagiumRich - RC helicopter (demo)Vincent - The athletic power of quadcopters Listener Pick of the Week Lindsay - Alcohol consumption and vaccines (Vaccine)Johnye - The Singing Microbiologist Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler The TWiVites read listener questions and comments about public engagement in science, vaccines, RNAi, reprogramming CD8 cells to treat cancer, rabies, and much more. Links for this episode Viral gifts Controlling plant viruses by vector control (Ann Rev Phyto) Flavivirus RNAi suppressor (J Virol) Entre probetas podcast (iTunes) Hedgehog central Reprogramming T cells for leukemia therapy (NEJM) Petri Dish podcast On Your Mind podcast Letters read on TWiV 264 Weekly Science Picks Kathy - Sorting algorithms visualizedAlan - StratodeanRich - Art in Science by Polyxeni PotterVincent - Pixel Genes Listener Pick of the Week Judi - Olympus BioScapes Winner Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, Kathy Spindler, and Ben tenOever Ben joins the TWiV team to reveal the winner of his contest in which influenza viruses carrying different interferon-stimulated genes vie against one another in mice. Links for this episode Virusninja Game of clones (Vax Rep) Game of clones bracket Letters read on TWiV 263 Weekly Science Picks Kathy - The Brain Scoop, esp. Where My Ladies AtAlan - Say 'hi' to a spaceship (YouTube)Rich - Garmin BlueChart Mobile and Active CaptainVincent - Journal boycott (one, two)Dickson - Futurescape TV show Listener Pick of the Week Luis - Spillover by David QuammenKen - Gravity Send your virology questions and comments (email or mp3 file) to [email protected]

Host: Vincent Racaniello Guest: Ann Palmenberg Vincent returns to the University of Wisconsin - Madison to speak with Ann Palmenberg about her career in virology. Links for this episode Palmenberg lab website - great resources Genome sequences of all rhinoviruses (J All Clin Imm) Cardioviral poly(C) tracts (Arch Virol Supp) EMCV disruption of nuclear transport (PNAS) Picornavirus protease in replicase gene (J Virol) Bacteriophage Qbeta replicase (J Virol) Thanks to Jordan for the episode title! Video of this episode - view at YouTube Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello and James Van Etten Guests: Chantal Abergel and Jean-Michel Claverie Vincent meets up with Chantal and Jean-Michel at the first International Symposium on Giant Virus Biology in Tegernsee, Germany, to discuss their work on Mimivirus, Megavirus, and Pandoravirus. Links for this episode Unanswered giant virus questions (Adv Virus Res) Fourth domain of life (Commun Integr Biol) Pandoraviruses (Science) Phaeocystis globosa virus (PNAS) Cafeteria roenbergensis virus (PNAS) Symposium group photo (jpg) Video of this episode - view at YouTube Send your virology questions and comments (email or mp3 file) to [email protected]

Host: Vincent Racaniello Guests: Tom Friedrich, Tony Goldberg, and David O'Connor Vincent visits the University of Wisconsin, Madison and speaks with Tom, Tony, and David about their work on virus discovery at the AIDS Vaccine Research Laboratory. Links for this episode A tick in Tony's nose (Am J Trop Med Hyg) Two new SIV from colobus monkeys in Uganda (Biomed Cent) Pegivirus diversity in Uganda (J Gen Virol) Simian hemorrhagic fever virus diversity in African primates (J Virol) Video of this episode - coming soon Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello and Rich Condit Guest: Jaquelin Dudley Vincent and Rich join Jackie at the University of Texas, Austin to talk about her work on mouse mammary tumor virus. Links for this episode Rem signal peptide processing and function (J Virol) MMTV null mice and tumorigenesis (J Virol) Jackie's publications (PubMed) I want my MMTV (TWiV 242) Live at ASV in Madison (TWiV 193) Symbiotic safecrackers (TWiV 154) Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, Kathy Spindler, and Matt Frieman Matt joins the TWiV team to discuss the discovery of a SARS-like coronavirus in bats that can infect human cells, and what is going on with MERS-coronavirus. Links for this episode SARS-like virus in bats binds ACE2 (Nature) Bat SARS-like virus that infects human cells (virology blog) Coronavirus infections update (WHO) Lack of MERS coronavirus antibodies in humans (EID) Mice not susceptible to MERS-CoV (J Gen Virol) MERS-CoV case imported into Spain MERS-CoV like virus in European hedgehogs (J Virol) DPP4 amino acids positively selected in bats (Virol J) Image credit: Richie Frieman Letters read on TWiV 258 Weekly Science Picks Kathy - Optical illusion (YouTube)Alan - AmboceptorMatt - Reply all by Richie Frieman (audio sample)Vincent - The microbiology of beer Listener Pick of the Week Cassie - NeatoShopPeter - Synapse evolution with Seth Grant (BSP 101) Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiV team consider how the kinase mTOR modulates the antibody response to provide broad protection against influenza virus, and explore the problems with scientific research. Links for this episode Two lives intertwined (Ann Rev Imm) John Holland, 83 Kinase mTOR modulates antibody response (Nat Imm) Drug widens immunity to flu (TheScientist) Concerto in B (TWiV 161) How science goes wrong (Economist) Trouble at the lab (Economist) Reforming science (TWiV 184) Letters read on TWiV 257 Weekly Science Picks Dickson - Tesla Motors superchargerKathy - FASEB contest: Stand out for scienceAlan - Natural History Museum virtual tourRich - Mauna Kea heavensVincent - FAA expands use of electronics on planes Listener Pick of the Week Peter - Score and ignore (pdf) Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Vincent, Dickson, Alan, Rich, and Kathy review two papers that present evidence for RNA interference as an antiviral immunity mechanism in mammals. Links for this episode: World Polio Day Mole Day John Holland's publications RNAi is antiviral in mammals (Science) Antiviral RNA interference in mammals (Science) RNAi, antiviral after all (Science) Is RNA interference antiviral in mammals? (Cell Host Microbe) Nodamura virus (Nature) Ebolavirus proteins suppress RNAi (J Virol) Illustrated is an siRNA (orange), dicer (top right), and argonaute (bottom) Letters read on TWiV 256 Weekly Science Picks Dickson - Wildlife Photographers of the Year 2013Kathy - John Holland's Emerging Infectious Disease lecture (YouTube)Alan - The worst part is notRich - The Universe in a Single Atom by Dalai Lama (Mind and Life Institute)Vincent - The Truth about T. Rex by Brian Switek Listener Pick of the Week Stephen - International Institute for Species Exploration (Top 10 species choice) Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello and Rich Condit Guests: Robert Krug and Christopher Sullivan Vincent and Rich visit the University of Texas at Austin and meet up with Bob and Chris to talk about their work on influenza virus and microRNAs. Links for this episode: Virus-encoded microRNAs (PLoS Path) microRNA targetomes of polyomavirus (J Virol) Innate and RNAi reciprocal inhibition (Cell) ISG15 pathway (Trends Micro) Role of viral NS1 protein (Virology) Cap-snatching (Cell) Weekly Science Picks Rich - Unraveling BoleroVincent - New botulinum toxin, DURC implications, and inconvenient truths Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Vincent, Dickson, Alan, and Kathy review clearance of simian immunodeficiency virus infection from macaques by immunization with SIV proteins encoded in a rhesus cytomegalovirus vector. Links for this episode: Immune clearance of SIV infection (Nature) Rhesus CMV vectors (Nature Medicine) Types of memory T cells (WikiPedia) Goldilocks approach to vaccines (Scilogs) Chimeric HA influenza vaccine (J Virol) Influenza vaccine matched vs mismatched (BMC Medicine) Letters read on TWiV 254 Weekly Science Picks Dickson - The Man Who Planted Trees by Jean GionoKathy - A boy and his atom and Moving Atoms (YouTube)Alan - It is pitch dark and Get Lamp (YouTube)Vincent - Who's afraid of peer review? (Science) Listener Pick of the Week Maren - Hello Maestro (episodes on YouTube)Ayesha - Why you don't f****** love scienceJon - A Capella Science (YouTube) Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler In this second consecutive all-email episode, the complete TWiV team reads questions and comments from listeners about systemic antiviral responses, wild type poliovirus in Israel, Turkish scientists, viral symbiotes, and much more. Links for this episode: One Nation in support of biomedical research? Bilim Kazani (The Cauldron of Science) Science for Gezi State of Turkish Science Pathogenesis of feline coronavirus (EID) Dinosaurs plagued by infection (PLoS One) Dinosaur renaissance (WikiPedia) Birds are dinosaurs (AMNH) Influenza vaccine enhances disease in pigs (Sci Transl Med) Gut microbes influence B-cell development (Nature) Circovirus in dogs (EID) We the microbiologist Letters read on TWiV 251 Weekly Science Picks Dickson - HD slow motion montage (YouTube)Kathy - Damselflies and Eyewitness appAlan - Space weather forecast (YouTube)Rich - 1984 by George Orwell (Doublethink)Vincent - PopSci comments off and Why so few women in science? Listener Pick of the Week Meika - Flip action roll (YouTube)CN - Blink: The Power of Thinking without Thinking by Malcolm GladwellBernadeta - Higgs Boson wins Nobel Prize Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The complete TWiV team reads email from listeners about anti-vaccine activists, a career in microbiology, placentas, a virology textbook, the HeLa cell genome, norovirus, and much more. Links for this episode: Anthropic principle 1999 vaccine article by Alan Dove (Nature) Image credit: Jason Roberts Letters read on TWiV 251 Weekly Science Picks Dickson - A Sand County Almanac by Aldo LeopoldKathy - Noctilucent clouds and aurora over ScotlandAlan - Flu vaccination mapRich - There's a fly in my urinalVincent - Picornaviridae.com Listener Pick of the Week Peter - PDB-101Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The complete TWiV crew reviews work on MERS-coronavirus, including serological studies in camels, production of an infectious DNA clone, and identification of an interferon antagonist. Links for this episode: Stephan Chron dies (NY Times) Philip Marcus dies (NY Times) Philip Marcus on TWiV #197 Ellen Fanning dies (news@Vanderbilt) Donald Low dies (CBC News) MERS timeline MERS-CoV seroepidemiology (Eurosurveillance) MERS-CoV neutralizing antibodies in camels (Lancet Inf Dis) MERS-CoV vaccine candidate (mBio) MERS-CoV infectious DNA (PNAS) MERS-CoV protein 4a is IFN antagonist (J Virol) Letters read on TWiV 251 Weekly Science Picks Dickson - Scharf PhotoKathy - Blaschka glass models (NY Times, Cornell)Alan - Digital Public Library of AmericaRich - Dengue virus life cycle animationVincent - Creative Live Listener Pick of the Week John - Last person to get smallpox (NPR)Peter - SARS documentary (BBC) Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello and Robert Garcea Vincent and Robert recorded this episode at the 53rd ICAAC in Denver, where they talked about polyomaviruses. Links for this episode: A cornucopia of human polyomaviruses (Nat Rev Micro) Polyoma assembly factories in nucleus (PLoS Path) Overprinting gene in Merkel cell polyomavirus (PNAS) Human JCV as population marker (PLoS One) Letters read on TWiV 250 Video of this episode (YouTube) Weekly Science Picks Robert - The Panic Virus by Seth MnookinVincent - Aliens chestburster behind the scenes Listener Pick of the Week Adam - Virology Fact of the DayChristophe - dr Karl Send your virology questions and comments (email or mp3 file) to [email protected]