This Week in Virology

Host: Vincent Racaniello Guest: Sandy Weller Vincent speaks with Sandy Weller about her career and her work on the mechanisms of synthesis, maturation and cleavage and packaging of viral DNA genomes. Become a patron of TWiV! Links for this episode Cell killing by avian leukosis virus (Sandy's first paper, J Virol) ND10 protein and herpes simplex virus replication (J Virol) Recombination promoted by DNA viruses (Ann Rev Micro) HSV-1 and DNA damage response (Fut Virol) Video of this episode at YouTube This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Send your virology questions and comments to [email protected]

Host: Vincent Racaniello Guest: David Tuller Vincent speaks with David Tuller about flaws in the U.K's $8 million PACE trial for chronic fatigue syndrome, and efforts to have the trial data released. Become a patron of TWiV! Links for this episode Science and journalism (TWiV 111) Chronic fatigue syndrome and the CDC (virology blog) PACE trial (Lancet) Trial by error part one, two, three (virology blog) PACE economics analysis (PLoS One) All articles by David Tuller at virology blog Valerie Eliot Smith's blog Karina Hansen, prisioner of Denmark This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Send your virology questions and comments to [email protected]

Host: Vincent Racaniello Guest: Peter Palese Vincent speaks with Peter Palese about his illustrious career in virology, from early work on neuraminidases to universal influenza virus vaccines. Become a patron of TWiV! Links for this episode Palese Laboratory Pig kidney neuraminidase (Hoppe Seylers Z Physiol Chem) DNAse in cytoplasmic DNA virus (Virology) Inhibitor of influenza virus neuraminidase (Virology) Influenza neuraminidase defective mutants (Virology) Swine influenza virus of 1976 RNA pattern (Nature) 1977 influenza H1N1 similar to 1950s strains (Nature) H5N1 influenza: Facts, not fear (PNAS) Influenza HA stalk immunity in ferrets (J Virol) This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Rich Condit, and Kathy Spindler Guest: Stephen J. Russell From ASV 2016 at Virginia Tech, Vincent, Rich and Kathy speak with Stephen Russell about his career and his work on oncolytic virotherapy - using viruses to treat cancers. Become a patron of TWiV! Links for this episode Hokies go viral (TWiV 285) MV NIS de Myelo (TWiV 298) Remission of disseminated myeloma after MV treatment (Mayo Clin Proc) miRNA detargeting (J Virol) Video of this episode at YouTube This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Weekly Science Picks Kathy - Can you explain your science in 30 seconds? and How Far Can We Go?Rich - Small asteroid Earth's companionVincent - The Gene by Siddhartha Mukherjee Listener Picks Dylan - Microsculpture by Levon Biss (website) Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello and Alan Dove Guest: Erica Ollmann Saphire Vincent and Alan speak with Erica about her career and her work on understanding the functions of proteins of Ebolaviruses, Marburg virus, and other hemorrhagic fever viruses, at ASM Microbe 2016 in Boston, MA. Become a patron of TWiV! Links for this episode Structure of antibodies bound to Ebola virus glycoprotein (PNAS) Structure of cross-reactive filovirus antibody (Cell) Multiple functions of Ebola virus VP40 protein (Cell) View video of this episode on YouTube Letters read on TWiV 394 This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Weekly Science Picks Alan - Slime LapseVincent - Virus Watch Listener Picks Patricia - NECSS, People Behind the Science, The Life Scientific Peter - Iron Lung on eBay Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler The TWiVerati revisit possible sexual transmission of Zika virus, and reveal how a cell protein that allows hepatitis C virus replication in cell culture enhances vitamin E mediated protection against lipid peroxidation. Become a patron of TWiV! Links for this episode Late sexual transmission of Zika virus (Lancet) Zika virus disease in US (CDC) Zika virus lab accident (ABC News) Mosquito eradication (virology blog) Florida mosquito control (TWiV 111) How tequila mosquito (TWiV 178) SEC14L2 allows HCV replication in cell culture (Nature) Lipid peroxidation (Wikipedia) HCV replicase regulated by lipid peroxidation (Nat Med) Letters read on TWiV 393 This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Also brought to you by ASV 2016 Weekly Science Picks Alan - All About That SpaceRich - The Expanse by James S.A. Corey (SyFy) Kathy - Five Pivotal Paragraphs in a PaperVincent - Agar Art Contest Winners Listener Picks Ted - Marshall Niremberg charts Send your virology questions and comments to [email protected]

Host: Vincent Racaniello Guests: Michael Diamond, Michaela Gack, Laura Kramer, and Charles Rice Four virologists discuss our current understanding of Zika virus biology, pathogenesis, transmission, and prevention, in this special live episode recorded at the American Society for Microbiology in Washington, DC. [powerpress url="http://traffic.libsyn.com/twiv/TWiV392.mp3"] Click arrow to play Download TWiV 392 (62 MB .mp3, 85 min) Subscribe (free): iTunes, RSS, email, Google Play Music Video of this episode at YouTube This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Alan Dove, and Rich Condit Guests: David Pride and Forest Rohwer If you have always wanted to know what coral reefs and the human oral cavity have in common, listen as guests David Pride and Forest Rohwer talk about their work on the microbiomes and viromes of these two environments, and you'll also understand why mucus is cool. Links for this episode Lytic to temperate switching of viral communities (Nature) Viromes in ancestral holobiont Hydra (PLoS One) Phage in human oral cavity (J Oral Micro) Human oral viruses are personal, consistent, gender specific (ISME) Video of this episode at YouTube This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Weekly Science Picks Alan - Vax Pack HeroRich - Helicopter autorotation Vincent - Live in Our Phage World by Forest Rohwer, Merry Youle, Heather Maughan, Nao Hisakawa Listener Picks Matt - Experiment.com Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guests: Ethan Jackson and Jonathan Carlson Project Premonition, a Microsoft Research project that uses drones to capture mosquitoes and analyze them for pathogens, preprint servers, and three mouse models for Zika virus induced birth defects are the topics of this episode hosted by the TWiumvirate and theirs guests Ethan and Jonathan. Links for this episode Project Premonition Premonition feasibility study in Grenada Zika virus causes microcephaly in mice (Cell Stem Cell) Brazilian Zika virus causes defects in experimental models (Nature) Zika virus causes placental damage and fetal demise in mice (Cell) Letters read on TWiV 390 This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Also brought to you by ASV 2016 Weekly Science Picks Dickson - Germany nearly reached 100% renewable power Alan - Vertical City documentary and Lumino City gameRich - Errol Morris: 'Demon in the Freezer' Kathy - Entropic Time YouTube by A Capella Science (watch "the making of")Vincent - Agar Art contest finalists Listener Picks Jennie - SciFi reading (scroll down) Send your virology questions and comments to [email protected]

Host: Vincent Racaniello Guest: Stan Lemon Vincent speaks with Stan Lemon about his career in virology, from early work on Epstein Barr virus, through making essential discoveries about hepatitis A virus, hepatitis C virus, and rhinoviruses. Links for this episode Live in Edmonton, Eh? (TWiV 235) Replication of EBV in epithelial cells (Nature) Returning to Carolina after 14 years Evolutionary origins of hepatitis A virus (TWiV 265) cre in rhinovirus genome (RNA) Naked viruses that aren't always naked (Ann Rev Virol) miR-122 and hepatitis C virus RNA (Cell Host Micr) This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Preprint servers, the structure of an antibody bound to Zika virus, blocking Zika virus replication in mosquitoes with Wolbachia, and killing carp in Australia with a herpesvirus are topics of this episode hosted by Vincent, Dickson, Alan, and Kathy. Links for this episode biorXiv preprint server Please review Vaccines and Your Child Structure of antibody bound to Zika virus E protein (Cell Host Microbe) Wolbachia blocks Zika virus in mosquitoes (Cell Host Microbe) Zimmer on Wolbachia and Zika virus (NY Times) Fauci on Zika virus vaccine (Science) Carpageddon (The Conversation) Letters read on TWiV 388 This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Also brought to you by ASV 2016 Weekly Science Picks Dickson - EarthEnv Alan - Research funding by lottery Kathy - Eugenia Cheng Math and BakingVincent - Zika Diaries Listener Picks Stephen - Virus trading cards William - Virus trading cards Norma+Maurice - Virus trading cards Tom - Virus trading cards Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Guest: Nina Martin Nina Martin joins the TWiV team to talk about the movie Vaxxed, her bout with dengue fever, and the latest research on Zika virus. Links for this episode Public Health United Fifty Shades of Science (TEDx) Parents convicted in death of toddler (Science Based Medicine) CDC: Zika virus causes microcephaly (NEJM) Dengue virus antibodies enhance Zika virus infection (biorXiv) Zika virus in Brazilian nonhuman primates (biorXiv) Zika virus IgM in CSF of microcephalic babies (Lancet) Brain organoids for modeling Zika virus infection (Cell) Letters read on TWiV 387 This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Also brought to you by ASV 2016 Weekly Science Picks Nina - Vaccines and Your Child by Paul Offit and Charlotte MoserDickson - The animals of Chernobyl Alan - Five rules of lab safety Kathy - 20 best science images of the year?Vincent - Massive undersea crab swarm Listener Picks JP - Global map of wind Todd - Doc Martin Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Rich Condit, and Kathy Spindler Guests: Ted Diehl and Welkin Johnson Ted and Welkin inform the TWiV team how the evolution of ancient retroviruses can be inferred by studying their sequences in the genomes of modern mammals, and join in a discussion of virus dispersal during different methods for drying hands. Links for this episode More fun than a monkey full of viruses (TWiV 122) Tracking ancient retrovirus evolution in mammals (eLife) Virus dispersal during hand drying (J Appl Micro) More than you wanted to know about hand drying (one, two, three, four, five, six) This episode is sponsored by ASM Agar Art Contest, ASM Grant Writing Course, and ASV 2016 Weekly Science Picks 1:30:20 Welkin - Lab Girl by Hope Jahren (essay)Ted - Accidental brilliance in scienceRich - Perpetual motion?Kathy - R0 graphic and explanationVincent - NWS ditches all caps forecasts Listener Picks Basel - Gimli Glider and Mars Climate OrbiterKen - Vaccination comic book Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello and Dickson Despommier Guest: Stuart Firestein Stuart joins Vincent and Dickson to talk about his sequel to Ignorance, a book called Failure, which seeks to make science more appealing by revealing its faults. Links for this episode Stuart Firestein's site Ignorance with Stuart Firestein (TWiV Special) The pursuit of ignorance (TED) Ignorance by Stuart Firestein Failure by Stuart Firestein This episode is sponsored by ASM Agar Art Contest and ASV 2016 Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove and Rich Condit Guests: Nischay Mishra and Ian Lipkin Nischay and Ian join the TWiVions to discuss mass die-offs of tilapia by a novel orthomyxo-like virus, Ian's editorial on the movie Vaxxed, and new vaccines to prevent dengue virus infections, including a human challenge model. Links for this episode Novel orthomyxo-like virus in Tilapia die-offs (mBio) Fish flu (TWiV 41) Anti-vaccination lunacy won't stop (WSJ) Single-dose dengue vaccine effective (J Inf Dis) Dengue vaccine needs (J Inf Dis) Composition of TV003 (J Inf Dis) Dengue vaccine mutagenesis (Curr Top Micr Imm) Dengue human challenge model (Sci Transl Med) Yellow fever alarm (STAT) Experiment: Zika virus UC Berkeley Microbiology Student Symposium Image credit Letters read on TWiV 384 This episode is sponsored by ASM Agar Art Contest, ASM Grant Writing Course, and ASV 2016 Weekly Science Picks Rich - Dune by Frank HerbertAlan - Adult vaccination video contestDickson - Ski town greenhouseVincent - Preprint servers: Force awakens or revenge of undead? Listener Picks Ken - Golden Mole Awards for Accidental Brilliance (list, video)Jim - Linear algebra course Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove and Kathy Spindler Guest: Esper Kallas Esper and the Merry TWiXters analyze the latest data on Zika virus and microcephaly in Brazil, and discuss publications on a mouse model for disease, infection of a fetus, mosquito vector competence, and the cryo-EM structure of the virus particle. Links for this episode Zika virus in the Americas (Science) Co-circulation of dengue, Zika, chik in Brazil (Medicine) Murine model for Zika virus infection (Am J Trop Med Hyg) Zika virus susceptibility of A. aegypti and A. albopictus (PLoS NTD) Infectious Zika virus from saliva (EuroSurveill) Zika virus spread through saliva? (Braz Oral Res) Five Zika virus reviews (one, two, three, four, five) Zika virus and microcephaly, Brazil (MMWR) Zika virus in urine (EuroSurveill) Zika virus infection of fetus (NEJM) Real-time sharing of Zika virus data (JAMA) Image credit: Richard Kuhn Letters read on TWiV 383 This episode is sponsored by ASM Agar Art Contest and ASV 2016 Weekly Science Picks Esper - The Chaos by Gérard Nolst Trenité and HIV pathogenesis courseAlan - Science KombatDickson - Four steps to wiping out a mosquitoVincent - Public lacks information on Zika virusKathy - Zika virus cryo-EM structure video Listener Picks Justin - Culture-independent discovery of Archaeal virusMelinda - Zika virus open research portal and Your body battles series Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove and Kathy Spindler Guests: Nels Elde and Edward Chuong Nels and Ed join the TWiV team to talk about their observation that regulation of the human interferon response depends on regulatory sequences that were co-opted millions of years ago from endogenous retroviruses. Links for this episode Endogenous retroviruses and regulation of innate immunity (Science) Placenta is viral (Nature) An ERV that maintains pluripotency of human embryonic stem cells (TWiV 279) Letters read on TWiV 382 This episode is sponsored by ASM Agar Art Contest and ASV 2016 Weekly Science Picks Nels - Don't Get Me Started - Matt GiorgianniAlan - Sun's magnetic field (paper)Dickson - Most realistic dinosaur terrifiesVincent - Top secret!Kathy - Zika virus risk for US 50 cities (paper)Ed - Watching comb jellies poop Listener Picks Asal - Lost paper of Gregor MendelDennis - PhysicsGirlJacob - Second (XKCD) and ACI blogger interview Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Carl Zimmer Carl Zimmer joins the TWiV team to talk about his career in science writing, the real meaning of copy-paste, science publishing, the value of Twitter, preprint servers, his thoughts on science outreach, and much more. Links for this episode Carlzimmer.com All Carl's articles at NYTimes Carl at Stat The Loom Staying afloat in the rising tide of science (Cell) Parasite Rex Planet of Viruses Handful of biologists went rogue (NYTimes) This episode is sponsored by ASV 2016 and ASM Agar Art Contest Weekly Science Picks Carl - Surprising benefit of viral DNAAlan - Global ship trafficDickson - Music with 2000 marblesVincent - The whiteboard revolutionKathy - How to interpret UK and American advisorsRich - Carina Storrs, science writer Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiVeroos deliver the weekly Zika Report, then talk about a cryoEM structure of a plant virus that reveals how the RNA genome is packaged in the capsid, and MIMIVIRE, a CRISPR-like defense system in giant eukaryotic viruses. Links for this episode First worms, then viruses, now dormant cyber pathogens (Zdziarski blog) Guillain-Barré with Zika virus in urine (Eurosurveillance) Zika virus in urine, Brazil (J Clin Virol) Zika in pregnant women in Rio (NEJM) Issues with NEJM pregnant women study (Epi-Ramble) Zika virus and meningoencephalitis (NEJM) Zika virus and acute myelitis (Lancet) Person to person transmission of Zika virus (MMWR) Pyrethroids and mouse microcephaly (one, two) Zika virus illustrations (Visual Science) Cowpea mosaic virus at high resolution (Nat Comm) MIMIVIRE confers resistance to virophage (Nature) CRISPR in a phage (TWiM 56) National Academy of Sciences GoFoc meeting Image credit Letters read on TWiV 380 This episode is sponsored by ASM Agar Art Contest and Microbe Magazine Podcast Weekly Science Picks Alan - Progression of EvolutionDickson - Toddler dies of meningitisVincent - Gardasil ideology, not scienceKathy - The Noun ProjectRich - Physics Girl Listener Pick Mark - MyRadar app (iOS, Android) Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Scott Tibbetts Scott Tibbetts joins the TWiVists to describe his work on the role of a herpesviral nocoding RNA in establishment of peripheral latency, and then we visit two last minute additions to the Zika virus literature. Links for this episode Noncoding RNA required for establishment of latency (mSphere) 17:20 Lessons learned from in vivo studies of a viral noncoding RNA (mSphere) 1:05:05 Zika virus in pregnant women in Rio de Janeiro (NEJM) 1:06:10 Zika virus infects cortical neural progenitors (Cell Stem Cell) 1:12:00 Image credit Letters read on TWiV 379 1:15:10 This episode is sponsored by 32nd Clinical Virology Symposium and Microbe Magazine Podcast 4:15, 1:05:25 Weekly Science Picks 1:42:45 Alan - Okeanos ExplorerDickson - Rome RebornVincent - Twitter Missing ManualScott - Best Science Images of 2015Kathy - Space Travel PostersRich - GoISSWatch Listener Pick Peter - Antibiotics and the Problem of the Broken Market Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Guests: Greg Smith and Mark Fuccio Greg Smith joins the TWiVirate to reveal how his lab discovered a switch that controls herpesvirus neuroinvasion, and then we visit the week's news about Zika virus. Links for this episode Dynamic ubiquitination drives herpesvirus neuroinvasion (PNAS) Releasing Zika virus data in real time (Nature) Zika virus infection and stillbirths (PLoS NTD) Zika virus infection, imported, Italy (Eurosurveillance) Letters read on TWiV 378 This episode is sponsored by 32nd Clinical Virology Symposium and Microbe Magazine Podcast Weekly Science Picks Alan - Science byline counting projectDickson - Bill Nye on climate changeVincent - Zdziarski's blog of things (Apple's motion to vacate)Greg - Atlas, the next generationKathy - Sources of Chaco woodMark - How Dogs Love Us by Gregory Berns Listener Pick Bohdan - The real reason for brains Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiVniks review the past week's findings on Zika virus and microcephaly, and reveal a chicken protein that provides insight on the restriction of transmission of avian influenza viruses to humans. Links for this episode Larvicide not involved in microcephaly (CBS News) Obama seeks $1.8b for Zika virus (Guardian) Zika virus in Puerto Rico (MMWR) Zika virus in semen (EID) Zika virus in Indonesia (EID) Phylogeny of Zika virus (EID) Zika virus may cause mental disease (NYTimes) Zika virus from amniotic fluid (Lancet) Host protein underlies avian influenza host restriction (Nature) Wendy Barclay on TWiV 177 Home to roost (Cell) Image credit Letters read on TWiV 377 This episode is sponsored by 32nd Clinical Virology Symposium and Microbe Magazine Podcast Weekly Science Picks Alan - CDC vaccine quizDickson - A telescope so powerfulVincent - Virology Lectures 2016 (virology blog, iTunes U)Rich - Ion propulsion (Wiki)Kathy - Spurious Correlations Listener Pick Johnye - Magellan telescope and van Egmond photograpy at Kids Should See This Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Jeremy Luban The TWiV team discusses the latest data on Zika virus, including ocular defects in infants with microcephaly, and isolation of the entire viral genome from fetal brain tissue. Links for this episode Ocular findings in infants with microcephaly (JAMA and Arq Bras Oft) Evidence of Zika virus infection in brain and placenta (MMWR) Zika virus associated with microcephaly (NEJM) Hard-core sequencing (Science) Zika virus and microcephaly (virology blog) Zika virus in the Americas (NEJM) JEV and WNV transplacental infections Rubella virus facts (WHO) Share your Zika virus reagents (virology blog) No link between anencephaly and Cache Valley Virus (EID) Image credit Letters read on TWiV 376 This episode is sponsored by 32nd Clinical Virology Symposium and ASM Microbe 2016 Weekly Science Picks Alan - PhD Comics on gravitational wavesDickson - 25 years of HubbleVincent - Amazon AWS TOS and Confirming EinsteinRich - Mosquito mass productionKathy - TileAppJeremy - IL17A and autism and The Perfect Theory by Pedro Ferreira Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler Guests: Carolyn Coyne, Helen Lazear, and Jeremy Luban The TWiVziks present everything you want to know about Zika virus, including association of infection with microcephaly and Guillain-Barré syndrome, transmission, epidemiology, and much more. Links for this episode Zika outbreak in Brazil (EID) 13:40 Zika summary (ProMedMail) 19:30 Zika and microcephaly (MMWR) 27:30 Facts about microcephaly (CDC) Brazil Ministry of Health microcephaly numbers Zika spread in Americas (MMWR) Deu Zica 42:30 Microcephaly in Brazil (Lancet) 46:10 GM mosquitoes did not start Zika outbreak (Discover) 1:31:30 Debunking myths around Zika (Mad Virologist) Sexual transmission vs sexually transmissible (virology blog) 1:22:10 CDC Zika travel guidance 1:15:45 CDC on Zika and sex 1:17:10 Image credit: Esper Kallas, University of Sao Paulo Letters read on TWiV 375 This episode is sponsored by 32nd Clinical Virology Symposium and ASM Grant Writing Webinar Weekly Science Picks 1:50:45 Alan - Jetstream Online School for WeatherVincent - New virus watercolors from Michele Banks (one, two)Rich - The Mad Virologist (Facebook, blog)Kathy - Orcas in AntarcticaJeremy - Pardis Sabeti on TEDHelen - Science ValentinesCarolyn - Three letter word missing from Zika warnings Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiVniks consider the role of a cell enzyme that removes a protein linked to the 5'-end of the picornavirus genome, and the connection between malaria, Epstein-Barr virus, and endemic Burkitt's lymphoma. Links for this episode Divergent requirements for removing VPg (mBio) Bond, covalent bond (TWiV 210) Link between malaria and endemic Burkitt's lymphoma (PLoS Path) Multifactorial role of malaria in Burkitt's lymphoma (PLoS Path) Plasmodium infection promotes AID-dependent B cell lymphoma (Cell) Children's cancer dependent on climatic factors (Nature) Denis Burkitt (Wikipedia) Request for PACE trial data (virology blog) Letters read on TWiV 374 This episode is sponsored by 32nd Clinical Virology Symposium and ASM Grant Writing Webinar Weekly Science Picks Alan - Indoor skydivingVincent - Cancer Virus by Crawford, Johannessen, and RickinsonRich - WitKathy - The Only Woman in the Room by Eileen PollackDickson - Show everyone your clinical data Send your virology questions and comments to [email protected]

Host: Vincent Racaniello Guest: Julius S. Youngner Vincent speaks with Julius about his long career in virology, including his crucial work as part of the team at the University of Pittsburgh that developed the Salk inactivated poliovirus vaccine. Links for this episode Trypsin for cell cultures (Proc Soc Exp Biol Med) Interferon induction by nonviral stimuli (J Gen Physiol) Interferons of different molecular weight (Proc Soc Exp Biol Med) Poliovirus thermal mutants (J Bacteriol) Combination of different cells in culture (Science) Equine influenza vaccine (Eq Vet J) Younger role in Salk vaccine (interview, amednews) Cutter Incident (JRSM) Video of this episode - coming as soon as I can get home! Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler The TWiV-osphere introduces influenza D virus, virus-like particles encoded in the wasp genome which protect its eggs from caterpillar immunity, and a cytomegalovirus protein counters a host restriction protein that prevents establishment of latency. Links for this episode Ebolavirus update (ProMedMail) Influenza D virus (mBio) Vincent's influenza C virus paper (J Virol) Virus-like particles protect wasp eggs (Sci Adv) CMV protein foils cell defense against latency (Sci Adv) Image credit: Mark Letters read on TWiV 372 This episode is sponsored by ASM Grant Writing Webinar and ASM Biodefense Weekly Science Picks Alan - Go viralVincent - 3D virus popsicles (3ders, dangerous popsicles)Rich - Galileo thermometerKathy - Thing Explainer by Randall Munroe Listener Picks Christiane - Using PowerPoint to shine Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiVologists discuss the finding of a second transmissible cancer in Tasmanian devils, and development of new poliovirus strains for the production of inactivated vaccine in the post-eradication era. Links for this episode Second transmissible cancer in Tasmanian Devils (PNAS) New strains for production of IPV (PLoS Path) Synchronized switch to bivalent OPV (polioeradication.org) Virologists, start your poliovirus destruction (virology blog) Image is a painting by George Kush Letters read on TWiV 371 This episode is sponsored by ASM Grant Writing Webinar and ASM Biodefense Weekly Science Picks Alan - Skyfaring by Mark VanhoenackerVincent - Your Cells. Their Research. Your permission?Rich - NASA UV photos of the sunKathy - Aurora bike lightsDickson - Unfilled vials Listener Picks Kevin - John Oliver on nutritional supplementsJim - Tour of Australian synchrotron Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler The TWiVomics review ten captivating virology stories from 2015. Ten virology stories from 2015 Recoding to make vaccines and study viral functions: TWiV 351: The dengue codeTWiV 362: Gotta catch ’em all and new paper Defining the virome: TWiV 365: Blood, feuds, and a foodborne disease, TWiV 362: Gotta catch ’em all, TWiV 356: Got viruses?, TWiV 355: Baby’s first virome, TWiV 342: Public epitope #1, TWiV 323: A skid loader full of viromes Ebola outbreak wanes, vaccines tested, persistence noted: TWiV 361: Zombie viruses on the loose, TWiV 349: One ring to vaccinate them all, TWiV 341: Ebolavirus experiences, TWiV 336: Brought to you by the letters H, N, P, and Eye, TWiV 335: Ebola lite Spillovers and scares: TWiV 347: Rose rosette and squirrel roulette, TWiV 351: The dengue code, TWiV 364: It’s not SARS 2.0, TWiV 365: Blood, feuds, and a foodborne disease The “Gain of Function” debate continues: TWiV 321: aTrip and a pause, TWiV 351: The dengue code, TWiV 354: The cat in the HAART, TWiV 363: Eat flu and dyad, and the NSABB’s new draft report Detailed mechanisms of pathogenesis: TWiV 336: Brought to you by the letters H, N, P, and eye, and TWiV 345: How a vaccine got the nod - plus numerous episodes (324, 328, 329, 330, 339, 343, 349, 353, 369) where we talked about basic research that undergirds these clinically important discoveries. Dengue vaccines: Trial TWiV 319: Breaking breakbone. Dengvaxia was licensed in December in Mexico, Philippines, and Brazil (12/28); paper we didn’t have time to cover about broadly neutralizing Abs from viremic patients and another paper from same group with X-ray crystal structure of Ab + gpE. Why do some RNA viruses undergo antigenic variation and others do not? TWiV 340: No shift, measles TWiV 346: A double helical career TWiV 345: How a vaccine got the nod Letters read on TWiV 370 This episode is sponsored by ASM Microbe and ASM Biodefense Weekly Science Picks Alan - Scientifics onlineVincent - Giant squid in Tokyo HarborKathy - A Year of Sky Listener Picks Daniel - Paper LifeStephen - How to deal with science denialistsVenkat - Kabisa Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler A swarm of virologists discusses testing of a MERS coronavirus vaccine for camels, and how a neuronal stress pathway reactivates herpes simplex virus. Links for this episode MERS coronavirus vaccine for camels (Science) WHO MERS summary Neuronal stress pathway and herpes simplex virus reactivation (Cell Host Micr) Stress flips a chromatin switch (Cell Host Micr) How herpes simplex virus reactivates (UNC press release) Histone antibodies database New antibody portal (UNC press release) Image credit Letters read on TWiV 369 This episode is sponsored by Clinical Virology Symposium Weekly Science Picks Alan - SpaceX Falcon 9 landingVincent - Scientists: Engage the public! and Lego contest winnersKathy - Google Ngram viewer Listener Picks Dennis - Americapox: The missing plagueMark - WeatherSpark and Quasispecies theory Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler A plaque of virologists explores the biology of Zika virus and recent outbreaks, and the contribution of a filamentous bacteriophage to the development of biofilms. Links for this episode More cowbell (Wikipedia) Microcephaly in Brazil (Outbreak News) Non-vector borne Zika transmission (EID) Zika virus outside Africa (EID) Zika virus possible sexual transmission (EID) Zika virus in saliva (J Clin Virol) Dengue's cousin Zika (Micr Inf) Filamentous phage promote biofilm assembly (Cell Host Micr) Biofilm history (MSU) Pseudomonas phage Pf1 Image credit Letters read on TWiV 368 This episode is sponsored by Clinical Virology Symposium and ASM Microbe Weekly Science Picks Alan - Twelve days of norovirusVincent - This Week in EvolutionRich - In the Kingdom of Ice by Hampton SidesKathy - Kajanjian lecture on Frederick Novy and Milestones ceremony at UM Listener Picks Peter - What if anti-vax, anti-science movement won?Stephen - Scandalous origin of vaccinesPritesh - Absurd way of doing business Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guests: Carolyn Coyne and Coyne Drummond Two Coynes join the TWiV overlords to explain their three-dimensional culture model of polarized intestinal cells for studying enterovirus infection. Links for this episode Carolyn and placentas on TWiV 193 Three-dimensional cell culture model (mSphere) Bioreactor video (JoVE) Neurovirologist Richard T. Johnson, 84 (Hopkins and virology blog) Tardigrade HGT disputed (BioRxiv) Exaptation of archaeal virus capsid protein from Cas4 (Biol Dir) Image credit Letters read on TWiV 367 This episode is sponsored by ASM Microbe Weekly Science Picks Kathy - Tardigrade genome sequence (video)Alan - Antibiotic action nonprofit groupVincent - Ex Machina and genome editing moratoriumRich - Launch photography by Ben CooperKathy - HIV life cycle in video (paper)Dickson - 2015 Nobel Lectures Physiology or MedicineCarolyn - Metapneumovirus entry Listener Picks Tom - Global host-pathogen databaseTrudy - Madame Curie by Eve Curie Send your virology questions and comments to [email protected]

Host: Vincent Racaniello Guests: Carla Giles, Zoe Dyson, Brianna McLean, and Caitlin O'Brien In Melbourne, Australia, Vincent speaks with four PhD students about their research projects and what it's like to get a doctorate down under. Video of this episode: view at YouTube This episode is sponsored by ASM Microbe and ASM Biodefense. Send your virology questions and comments to [email protected] TWiV is a MicrobeTV Production

Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler For a TWiV Thanksgiving, Vincent, Alan, and Kathy trace the feud over genome editing, a new virus discovered in human blood, and the origins of hepatitis A virus. Links for this episode Battle over genome editing (Wired) 10:05 First description of CRISPR in E. coli (J Bacteriol) 14:00 Cas genes identified (Mol Micro) 14:20 CRISPR-Cas provides immunity to bacterial viruses (Science) 15:35 Cas9-crRNA complex mediates DNA cleavage (PNAS) 16:55 Dual RNA-guided DNA endonuclease in bacterial immunity (Science) 17:50 Genome engineering using CRISPR/Cas (Science) 18:50 Cpf1 endonuclease (Cell) 23:10 Classification of CRISPR/Cas systems (Nat Rev Micro) 23:50 Summit on gene editing 21:25 Novel human virus in transfusion recipients (mBio) 27:00 Origins of hepatitis A virus in small mammals (PNAS) 55:05 Image credit Letters read on TWiV 365 7:00, 1:20:35 This episode is sponsored by ASM Microbe, ASM Biodefense, and the 32nd Clinical Virology Symposium. (1:10, 1:18:30) Weekly Science Picks 1:36:30 Kathy - Tardigrade genome sequence (video)Alan - XKCD on Gates polio eradication siteVincent - Lifting the Impenetrable Veil by Charles Calisher Listener Picks Judi - Jennifer Doudna's Ted TalkRamon - Map of the Sky Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Rich Condit, and Kathy Spindler Guests: Ralph Baric and Vineet Menachery Vincent, Rich, and Kathy speak with Ralph and Vineet about their research on the potential of SARS-like bat coronaviruses to infect human cells and cause disease in mice. Links for this episode Zoonotic potential of SARS-like bat viruses (Nature) SARS-CoV susceptibility loci via collaborative cross (PLoS Genetics) 47:00, 57:25 Ebright and Wain-Hobson quotes (Nature) 1:17:20 Misleading press release (UNC) 1:24:45 Super powerful SARS 2.0 (not!) (Motherboard) SARS-like bat virus can jump without mutating (not!) (Daily Mail) 1:26:30 Photo credit Letters read on TWiV 364 1:47:40 This episode is sponsored by Mt. Sinai Department of Microbiology (2:10), ASM Education (33:25), and the 32nd Clinical Virology Symposium (1:51:30) Timestamps by Jennifer. Thanks! Weekly Science Picks 1:53:00 Kathy - California Dept of Public HealthRich - Leafy greens on space stationVincent - Mechanical keyboards and TWiV on Perfect your podcast Listener Picks Jesse - Networking for Nerds by Alaina G. LevineRussell - Worldmapper Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler The TWiVers reveal influenza virus replication in the ferret mammary gland and spread to a nursing infant, and selection of transmissible influenza viruses in the soft palate. Links for this episode Photos of my visit to OHSU (Facebook) 6:30 Folta departs (Facebook) 9:25 T-VEC approved for melanoma (FDA) 14:45 Talimogene laherparepvec (Wikipedia) 15:10 T-VEC phase III trial (J Clin Oncol) 17:35 How drugs are named (PopSci) 16:35 Influenza transmission in mother-child dyad (PLoS Path) 23:10 Soft palate and influenza transmission (Nature) 41:00 Blausen 0872 UpperRespiratorySystem" by BruceBlaus. When using this image in external sources it can be cited as:Blausen.com staff. "Blausen gallery 2014". Wikiversity Journal of Medicine. DOI:10.15347/wjm/2014.010. ISSN 20018762. - Own work. Licensed under CC BY 3.0 via Commons." target="_blank">Image credit Letters read on TWiV 363 1:09:30 This episode is sponsored by Mt. Sinai Department of Microbiology, ASM Education, and ASM Biodefense and Emerging Diseases Conference Timestamps by Jennifer. Thanks! Weekly Science Picks 1:29:00 Kathy - Insect gearsAlan - Bye, Bye, EbolaRich - Hedy LamarrVincent - Should we resurrect extinct species? Listener Picks Jennie - Bye, Bye, EbolaPeter - Epidemics on edX Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler With their usual verve, the virus virtuosos illuminate a new method to identify all the viral nucleic acids in a sample, and regulation of viral gene expression by codon usage. Links for this episode Virome capture sequencing (mBio) 15:40 Codon usage regulates viral gene expression (PNAS) 40:00 Harold S. Ginsberg (Wikipedia) 7:15 Letters read on TWiV 362 6:50, 1:03:20 This episode is sponsored by ASM Education: ASM MOOC 5:45 Timestamps by Jennifer. Thanks! Weekly Science Picks 1:39:20 Kathy - Adam Savage, Andy Weir and Chris Hadfield talk about The MartianAlan - Project Apollo photo archive - and videoRich - Watch A Bowling Ball And Feather Falling In A VacuumDickson - California droughtVincent - Does AAAS care about Ebola anymore? Listener Picks Anonymous - Lives saved by vaccinationPaul - Science articles: A guideJudi - How a virus invades your body Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Rich Condit The TWiVsters discuss Frederick Novy's return from retirement to recover a lost rat virus, and evidence for persistence of Ebolavirus in semen. Links for this episode Frederick Novy and the rat virus (Ann Int Med) 18:20 The rat virus (J Inf Dis) 22:55 Recovery of the rat virus (J Inf Dis) 35:20 Ebola sitrep 42:25 Meningitis in Glaswegian Ebolavirus nurse (ProMedMail, Medscape) 45:50 Sexual transmission of Ebolavirus (NEJM) 50:25 Ebolavirus RNA persistence in semen (NEJM) 1:04:25 Airplane banner falls into Hudson River (jpg) Image credit Letters read on TWiV 361 7:25, 1:13:25 This episode is sponsored by ASM Education: ASM MOOC 3:50 Weekly Science Picks 1:27:05 Alan - StickyRich - Arthur C. Clarke predicts the Internet (so did Vannevar Bush)Dickson - Nikon Small World Photomicrography winnersVincent - Pat Schloss on microbiome initiative Listener Picks Awkward Skeptic - 16 year old wins Google prize for detecting Ebolavirus Timestamps by Jennifer. Thanks! Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello and Kathy Spindler Guests: Michael Imperiale, Adam Lauring, and Akira Ohno Vincent visits the University of Michigan where he and Kathy speak with Michael, Adam, and Akira about polyomaviruses, virus evolution, and virus assembly, on the occasion of naming the department of Microbiology & Immunology a Milestones in Microbiology site. Links for this episode World Polio Day 3:20 Mole Day 5:25 The rat virus (J Inf Dis) 20:00 Polyomavirus miRNAs (Curr Op Virol) 39:30 Costs of GOF pause (mBio) 45:00 Lethal mutagenesis of influenza virus (J Virol) 1:03:35 Mutational robustness in RNA virus evolution (Nat Rev Micro) 52:00 Membrane binding of gag (mBio) 1:12:55 Cell proteins in retrovirus particles (J Virol) 1:23:15 Michael's twitter Adam's twitter Video of this episode - view below or at YouTube Weekly Science Picks 1:27:30 Kathy - Ada Lovelace and Charles Babbage Lego #1 and #2 and Ada Lovelace DayVincent - MiSciWriters Listener Picks Junio - XKCD marks the spotKari - Spoonflower Send your virology questions and comments to [email protected]

Host: Vincent Racaniello Guest: Blossom Damania Vincent speaks with Blossom about her laboratory's research on Kaposi's sarcoma-associated herpesvirus, including how it transforms cells, the switch between lytic and latent replication, and its interaction with the innate immune system of the host. Links for this episode Damania Laboratory Modulation of cGAS-STING by KSHV (PNAS) KSHV vIRF1 interacts with ISG15 pathway member (J Virol) KSHV NLR homolog inhibits inflammasome (Science) KSHV inhibition of PI3K and mTOR (Blood) KSHV confers survival advantage to endothelial cells (Cancer Res) UNC Global Oncology Program Blossom on Twitter Video of this episode - view at YouTube Send your virology questions and comments to [email protected]

Host: Vincent Racaniello Guest: Ileana Cristea Vincent meets up with Ileana at Princeton University to talk about how her laboratory integrates molecular virology, mass spectrometry-based proteomics, and bioinformatics to unravel the interplay between virus and host. Links for this episode Cristea Laboratory Ileana's work previously on TWiV 269 Blowing off steam (Cell Host Micr) Nuclear viral DNA sensors (J Biol Chem) mSystems Video of this episode - view at YouTube (coming soon!) Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Jens Kuhn Jens joins the TWiVomics to discuss the strengths and weaknesses of viral taxonomy, including its history and evolution, how viruses are ordered, and why T. rex was classified without having a living isolate. Links for this episode Jens' previous TWiV (283) 5:15 International Committee on the Taxonomy of Viruses (ICTV) 27:50 ICTV 9th Report 32:55 Classifying viruses by capsid architecture (Curr Opin Virol) 1:43:00 Image credit Letters read on TWiV 357 10:55, 1:55:40 Timestamps by Jennifer. Thanks! Weekly Science Picks 56:30, 2:07:15 Alan - The Jefferson GridRich - Summer of ScienceKathy - Bill Nye dances The ClickDickson - The MartianJens - How I Killed Pluto, Life in Our Phage World, Why the Seahorse Tail is SquareVincent - Does the public trust science? (videos, summaries) Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Stephanie Neal Stephanie joins the super professors to discuss the gut virome of children with serious malnutrition, caterpillar genes acquired from parasitic wasps, and the effect of adding chemokines to a simian immunodeficiency virus DNA vaccine. Links for this episode Invitrogen Science Hero Awards 15:05 Gut DNA viromes of twins with severe malnutrition (PNAS) 34:30 Random forests 48:00 Wasps engineer caterpillars (PLoS Genetics) 50:20 Chemokine-adjuvanted SIV DNA vaccine (Mucos Immunol) 1:02:20 Guide to chemokines (pdf) Role for CCR10 in IgA secreting cell accumulation (J Immunol) CCL28 controls IgA plasma cell accumulation (J Exp Med) In vivo electroporation 1:07:25, 1:09:50 Kathy's groan (jpg) Image credit Letters read on TWiV 356 14:55 Weekly Science Picks 1:18:35, 1:45:30 Alan - Plates from Hooke's MicrographiaRich - The Wright Brothers by David McCulloughKathy - Spore evaporation-driven engines (video and article)Dickson - Color variations of Pluto and Lunar total eclipseStephanie - The science of moldy cheese by Carl ZimmerVincent - But Why? Listener Pick of the Week Reed - The Bench Warmers Podcast Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiV team considers the effect of a Leishmaniavirus on the efficacy of drug treatment, and the human fecal virome and microbiome in twins during early infancy. Links for this episode Announcing mSphere 5:50 Leishmania virus influences drug outcome (paper 1, paper 2) 25:15 Human gut virome and microbiome in infants (Nat Med) 46:00 Multiple displacement amplification (Wiki) 46:00 Virome of adult twins (Nature) 49:15 Illumination by Kevin Folta 1:31:00 Folta story at RetractionWatch 1:41:40 Image credit Letters read on TWiV 355 9:40, 1:20:30 This episode is sponsored by ASMGAP 5:00 Timestamps by Jennifer. Thanks! Weekly Science Picks 1:42:20 Alan - Vexed Muddler storeRich - Ivanpah Solar Power FacilityKathy - The real KermitDickson - Animal PortraitsVincent - Adventures of the Regatjes, Vaccines by Susan Nasif Listener Pick of the Week Jacob - Great Wall of China keeps rabbits out Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The esteemed doctors of TWiV review a new giant virus recovered from the Siberian permafrost, why influenza virus gain of function experiments are valuable, and feline immunodeficiency virus. Links for this episode Non-viral proteins in vaccinia virus particles (Arch Virol) 8:15 Thing a week (Jonathan Coulton, downloads) 23:20 Immortal Life pornographic? Not! (HuffPost) 25:25 Mollivirus sibericum (PNAS) 27:00 Don't worry about Frankenvirus (WashPost) 27:10 We need flu GOF research (article, Wisc State J, Science) 40:35 Isolation of FIV (Science) 50:20 FIV at Cornell Vet 1:06:25 FIV latency (Retrovirol) 1:08:00 FIV-receptor interactions (Curr Op Virol) 1:08:45 Clin aspects FIV infection (Viruses) FIV antivirals (Viruses) 1:10:05 How to treat FIV 1:10:45 Bud's FIV therapy 1;10;50 FIV vectors (CSH Prot) 1:13:50 Lion FIV (Proc Biol Sci) FIV in cat species (J Virol) 1;01;45 ACIP HVP vaccine recommendations (MMWR) 1:28:45 HPV and cancer (CDC) 1:33:45 Cervical cancer screening (pdf, CDC) Letters read on TWiV 354 7:45, 1:17:10 This episode is sponsored by ASMGAP Timestamps by Jennifer. Thanks! Weekly Science Picks 1:40:40 Alan - Experience CuriosityRich - Primrose SchoolKathy - Egg drop solutionsDickson - Dragonflies by Pieter van DokkumVincent - NASA guide to air-filtering houseplants Listener Pick of the Week Amir - Publons (Nature article) Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler The TWiVniacs discuss twenty-eight years of poliovirus shedding by an immunodeficient patient, and packaging of the innate cytoplasmic signaling molecule cyclic GMP-AMP in virus particles. Links for this episode 28 years of poliovirus shedding (PLoS Path) 36:50 NOT a rare poliovirus (Wash Post) 44:35 Ukraine polio outbreak (ProMedMail) 41:00 Virus particle transfer of cGAMP (Science one, two) 47:50 cGAS on TWiV 222 47:05 Image credit Letters read on TWiV 353 6:30, 1:10:25 This episode is sponsored by ASMGAP 3:10 Timestamps by Jennifer. Thanks! Weekly Science Picks 1:27:50 Alan - Miniature calendarKathy - Dr. Brainlove (prologue, v. 2.0, IndieGoGo)Dickson - The Ph.D. MovieVincent - Ghostery Listener Pick of the Week Ramon - Children dieChris - ASMLive at ICAAC/ICC Send your virology questions and comments to [email protected]

Host: Vincent Racaniello Guest: Michele Banks Vincent meets up with Michele Banks in Washington, DC to discuss her career as a creator of science-themed art. Links for this episode Michele Banks on Twitter Artologica Michele's blog The Finch and the Pea Joseph Cornell Not Exactly Rocket Science (Ed Yong) Tree of Life (Jonathan Eisen) Home Microbiome Study Kitten Microbiome Project Science Online The Vexed Muddler Luke Jerram A Daily Dish (Klari Reis) Neuroscience art (Greg Dunn) Ai Weiwei Video of this episode - coming soon Video of Michele working in her studio - view at YouTube Send your virology questions and comments to [email protected]

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The Masters of the ScienTWIVic Universe discuss a novel poxvirus isolate from an immunosuppressed patient, H1N1 and the gain-of-function debate, and attenuation of dengue virus by recoding the genome. Links for this episode Paula Pitha-Rowe (Balt Sun) Novel poxvirus (CID) H1N1 and gain-of-function debate (mBio) Recoding dengue virus genome (PNAS) Attenuation by dinucleotide frequency change (PNAS) Codon pair and dinucleotide bias not distinguished (PNAS) Codon pair deoptimization artefact of CG/UA change (eLife) Can engineering subdue viral vaccine reversion? (Virus Evol) Attenuation by a thousand cuts (NEJM) Image credit Letters read on TWiV 351 Weekly Science Picks Alan - Genetic roulette Rich - The Huntington Kathy - Simulated vomiting machine (video) Dickson - Last Cassini Saturn flybyVincent - Decaying Still Life (YouTube) Listener Pick of the Week Ken - Cave meterology (Times, Nature) Send your virology questions and comments to [email protected]

Host: Vincent Racaniello Guest: Katherine A. High Vincent speaks with Katherine High about her career and her work on using viral gene therapy to treat inherited disorders. This episode is drawn from one of twenty-six video interviews with leading scientists who have made significant contributions to the field of virology, part of the new edition of the textbook Principles of Virology. Links for this episode Katherine A. High, MD AAV gene therapy for congenital blindness (Sci Trans Med) Gene therapy for hemophilia (Hematol) AAV capsid decoys (Sci Trans Med) Video of this episode - view at YouTube Send your virology questions and comments (email or mp3 file) to [email protected]

Hosts: Vincent Racaniello, Alan Dove, and Rich Condit Vincent, Alan and Rich explain how to make a functional ribosome with tethered subunits, and review the results of a phase III VSV-vectored Ebolavirus vaccine trial in Guinea. Links for this episode Social media specialist position at ASM WHO dismisses Catholic church on vaccine safety U of T Dean resigns over anti-vaccine course (Tor Star) Vaccine Adverse Event Reporting System (VAERS) Countering anti-vaccination attitudes (PNAS) Designer ribosome (Nature) rVSV-EBOV preliminary report (NEJM) rVSV-EBOV phase III interim report (pdf, Lancet) World on verge of Ebolavirus vaccine (WHO) Ebolavirus vaccine in Africa (virology blog) Letters read on TWiV 349 11:55 This episode is sponsored by ASM GAP Weekly Science Picks Alan - Above and BeyondRich - DSCOVR (Dark moon side crossing Earth)Vincent - Stuxnet virus (Wired, YouTube) Listener Pick of the Week Konrad - Stated Clearly Send your virology questions and comments (email or mp3 file) to [email protected]