This Week in Virology

The TWiVome deconstructs the evolutionary history of RNA viruses, and immune promortion of murine norovirus pathogenesis by replication in intestinal tuft cells. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASM Microbe 2018 Support Viruses & Cells Gordon Conference Faculty positions at Icahn School of Medicine Evolutionary historyof RNA viruses (Nature) The amphioxus song Norovirus tropism for tuft cells(Science) Letters readon TWiV 491 Weekly Science Picks Alan - Oligodendroglioma in song Kathy- Everything-repellent coating to grime proof phone screens & more Rich- Gaia star map Dickson- Clouds are complicated Vincent - Eradicating polio with a vaccine we must stop using Listener Pick Stephen- Forget the Avengers, it took real heroes to make India polio-free Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From the TWiV team, human cowpox infection possibly acquired from a pet cat, and a new giant mimivirus of green algae with genes encoding enzymes of fermentation. Hosts: Vincent Racaniello, Dickson Despommier, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASM Microbe 2018 ASV Job Site revamped Support Viruses & Cells Gordon Conference Faculty positions at Icahn School of Medicine Dick Courtney obit Human cowpox infection(NEJM) Mimivirus encodes fermentation genes(Virology) Letters readon TWiV 490 Weekly Science Picks Kathy- Landscapes that look like another world (but they are from Spain) Rich - Brighton Collaboration; Viral Vector Vaccines Safety Working Group (V3SWG)(Chen & DeStefano: Vaccine adverse events: causal or coincidental?) Dickson- Clouds of Jupiter and EBNA2 and autoimmunity Vincent - More than one-third of graduate students report being depressed (original article) Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent visits Sandra Urdaneta-Hartmann at Drexel University College of Medicine in Philadelphia to talk about the development of the mobile video game 'CD4 Hunter'. Host: Vincent Racaniello Guest: Sandra Urdaneta-Hartmann Become a patron of TWiV! Links for this episode CD4 Hunter iOSAndroid Sandra on Twitter Carla Brown on Twitter Inactivation of HIV in breast milk(Retrovirology) Nipple shieldto inactivate HIV (Int J Pharm) Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV team reveals that recent mumps virus outbreaks in the US are due to waning vaccine efficacy, and an intranasally delivered small interfering RNA that controls West Nile infection in the brain. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Become a patron of TWiV! Links for this episode ASM Microbe 2018 ASV 2018 Microbiology Student Symposium at UC Berkeley Support Viruses & Cells Gordon Conference Waning mumps vaccinein US (Sci Trans Med) siRNA controlof West Nile virus brain infection (Cell Host Microbe) Image credit Letters readon TWiV 488 Weekly Science Picks Kathy- Peak bloom and photographing cherry blossoms Dickson- Origins of vertebrate RNA viruses Alan- Rare footage of anglerfishin the deep ocean Vincent - NIH must reduce disparities in funding Listener Picks Mariann - Eden Project and Bellyvision Apparatus and the Like Amir- Winners of ICR Science Photography and Photography Contest Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

At the Medical College of Wisconsin, Vincent talks with current and former members of the Department of Microbiology and Immunology about their work and their careers. Host: Vincent Racaniello Guests: Dominique Carter, Sid Grossberg, John Kirby, Scott Terhune, and Vera Terakanova Become a patron of TWiV! Intro music by Ronald Jenkees Send your virology questions and comments to [email protected]

Sean Whelan returns to TWiV to speak with Vincent about using haploid cell lines to identify genes encoding cell receptors for viruses, including Ebolavirus, Lassa virus, and more. Host: Vincent Racaniello Guest: Sean Whelan Become a patron of TWiV! Links for this episode Ebola virus entry requires the cholesterol transporter Niemann-Pick C1 (Nature) Lassa virus entry requires a trigger-induced receptor switch (Science) Infectious Entry Pathway Mediated by the Human ERV K Envelope Protein (J Virol) Novel Broad-Spectrum Virus Entry Inhibitor (J Virol) Mechanism of membrane fusion induced by VSV G protein (PNAS) NRP2 and CD63 Are Host Factors for Lujo Virus Cell Entry (Cell Host Micr) Image credit Intro music by Ronald Jenkees Send your virology questions and comments to [email protected]

The TWiV posse considers viral insulin-like peptides encoded in fish genomes, and insect antiviral immunity by production of viral DNA from defective genomes of RNA viruses. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Kathy Spindler, and Brianne Barker Become a patron of TWiV! Links for this episode ASM Microbe 2018 Insulin-like peptides in Iridovirus genomes (PNAS) Dicer-2 dependent generation of cvDNA from defective genomes (Cell Host Micr) Carla Saleh on insect antiviral immunity (TWiV 301) RNAseIII ancient antiviral RNA platform (TWiV 450) cvDNA precursor to EVEs (TWiV 482) Image credit: Paul Young Letters read on TWiV 485 Weekly Science Picks Kathy - Vegas casinos lost money on physicists Brianne - Astronaut twin's DNA is not 7% different! Dickson - Parasitic Diseases lecture videos Alan - In Season blog by Donna Long Vincent - Buckyball viruses Listener Picks Paul - Wine labels (front, back) Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiVumvirate discuss the giant Tupanvirus, with the longest tail in the known virosphere, and dampened STING dependent interferon activation in bats. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Kathy Spindler, and Brianne Barker Become a patron of TWiV! Links for this episode ASM Microbe 2018 Tailed giant Tupanvirus (Nat Commun) Soda Lakes (Wikipedia) Dampened STING in bats (Cell Host Microbe) Image credit Letters read on TWiV 484 Weekly Science Picks Kathy - How to read a phylogenetic tree Brianne - No immune overload for vaccines Dickson - Flowchart of viral families Alan - Letters to a Pre-Scientist Vincent - True believers, entrepreneurs, and scammers in alternative medicine and Simply Put: Vaccines Save Lives Listener Picks Vinayaka - Code of Ethics for Young Scientists Matt - TWiV 443 Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent and the Virals review undermining of antiviral effectiveness by genital inflammation, and heterogeneity of influenza virus infection in single cells. Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler Become a patron of TWiV! Links for this episode USA Science and Engineering Festival Genital inflammation and antiviral effectiveness (Nat Med) Single cell infection heterogeneity (eLife) Single-cell poliovirus infection (Virology) Image credit Letters read on TWiV 483 Weekly Science Picks Kathy - Dance Dance Rich - Snopes Alan - Next Generation Science Standards Vincent - TWiEVO 28: Genetics of skin pigmentation in Africa Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV Masters discuss serologic evidence of Ebolavirus infection in a population with no outbreaks, and the set of endogenous viral elements in the mosquito genome. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Kathy Spindler, and Brianne Barker Become a patron of TWiV! Links for this episode USA Science and Engineering Festival Ebolavirus infection without an outbreak (J Inf Dis) A. aegypti EVEome (Curr Biol) One Loop to Rule Them All (Trends Biochem Sci) Image credit Letters read on TWiV 482 Weekly Science Picks Kathy - Origami sound barrier Brianne - iBiology Dickson - Switched-on bats Alan - Hawaii's communication breakdown Vincent - Inside the Federal Bureau of way too many guns and What I Saw Treating Parkland Victims Listener Pick Johnye - Debunking Anti-Vaxxers Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiVodrome considers the intestinal tract as an alternative infection route for MERS coronavirus, and how reduced accumulation of defective viral RNAs might lead to severe influenza. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode HaHa influenza virus (mBio) MERS coronavirus intestinal transmission (Sci Adv) Simulated gastric and intestinal fluids Stomacher Defective genomes and severe influenza (PLoS Path) Image credit Letters read on TWiV 481 Weekly Science Picks Kathy - CD45.1 and CD45.2 mice may not be functionally equivalent Rich - Relative humidity versus dewpoint (video) Dickson - Underwater photography winners Alan - Photo of a single atom Vincent - Proposed Federal science budget cuts and The Blog is Dead Listener Pick Jess - Louis Tompkins Wright Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Scott Hensley joins the TWiVites to review the current influenza season and presence of the virus in exhaled breath of symptomatic cases. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Scott Hensley Become a patron of TWiV! Links for this episode Weekly influenza surveillance (CDC) Influenza vaccination status (CDC) H3N2 glycosylation site and eggs (PNAS) Influenza virus in exhaled breath (PNAS) Geometric Defects and Icosahedral Viruses (Viruses) Image credit Letters read on TWiV 480 Weekly Science Picks Kathy - Flow Virometry Rich - Falcon Heavy Launch links: Flight diagram; Launch including booster landing; Tesla in space; Tesla orbit; Reusable booster failures; Booster size Dickson - Next generation of weapons Alan - Ed Yong's story on fixing gender imbalance in science reporting Vincent - CD4 Hunter iOS Android Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiVome explores induction of antiviral responses by repeating patterns of capsids, and a fungus in the mosquito gut that aids dengue virus replication. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode Innate immune detection of capsid patterns (mBio) Mosquito gut fungus aids dengue virus replication (eLife) Nominate MicrobeTV for a Keck award Image credit Letters read on TWiV 479 Weekly Science Picks Kathy - Great Lakes, false color Rich - Times 360 Lego factory tour Dickson - 3D printed houses Alan - The new terms for trust in journalism Vincent - A Tale of Two Cultures and Why Science Blogging Still Matters Listener Picks Jolene - Immune Quest Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV team explains how infectious horsepox virus - likely the ancestor of smallpox vaccines - was recovered from chemically synthesized DNA fragments. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu Potential Zika virus related birth defects, USA (MMWR) Bordering on an outbreak (Tex Obs) Horsepox virus from synthesized DNA (PLoS One) Horsepox based smallpox vaccine (NEJM) Has horsepox become extinct? (Vet Rec) Evolutionary path of smallpox vaccines (Lancet Inf Dis) Equination (Vaccine) Image credit Letters read on TWiV 478 Weekly Science Picks Kathy - UM EEB photo contest Rich - Dino-Lite digital microscope and digital microscope camera Dickson - Sandford Photography Alan - A list of female virologists Vincent - Virology Lectures 2018 Listener Picks Andrew - Interferome Neva - The Untreatable by Gavin Francis Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiVodrome explains how a gag-like protein from a retrotransposon forms virus-like particles that carry mRNA within vesicles across the synapse. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu Arc virus-like particles transport RNA across the synapse (mammalian, Drosophila) Chemical synapse (Wikipedia) 2 minute video on the neuron Image credit Letters read on TWiV 477 Weekly Science Picks Kathy - Ice cliffs form over the weekend Rich - The Courage To Invent: A NASA Roboticist Tells Her Story (Ayanna Howard) Dickson - 2017 Was One of the Hottest Years on Record Alan - Experimenter gender and replicability Vincent - Set Your Autocorrect to Change 'Guys' to 'Folks' Listener Picks Asal - 100 million chickens with avian flu Mark - Dr. Tatiana's Sex Advice to All Creation Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Nels joins the TWiV team to talk about his work on genomic accordions in vaccinia virus, hepatitis B virus in a 439 year old mummy, and viral induction of energy synthesis by a long noncoding RNA. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Nels Elde Click arrow to play Download TWiV 476 (63 MB .mp3, 105 min) Subscribe (free): iTunes, RSS, email Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu TWiV is a must-listen (WaPo) More on poxvirus accordions (bioRxiv) HBV from a 439 year old mummy (PLoS Path) lncRNA promotes viral replication by inducing metabolism (Science) Making Data Visual Letters read on TWiV 476 Weekly Science Picks Nels - Everyday Evolution Kathy - Paper-fold an ellipse Rich - Oxford Nanopore Technologies: YouTube channel; General technology; DNA sequencing; Sequencing singularity Dickson - Spiders that look like pelicans Alan - Guessing pool for China Spacelab reentry Vincent - How to take a picture of the stealth bomber over the rose bowl Listener Picks Gretchen - The Bearded Lady Project Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

In the first episode for 2018, the TWiV team reviews the amazing virology stories of 2017. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu Virology stories of 2017 - in no particular order A virologist in space - TWiV 444 Cool things viruses do: Astrovirus capsids are enterotoxins TWiV 423; giant Klosneuviruses encode lots of stuff for translation TWiV 437; Giant Noumeaviruses quickly make the nucleus temporarily leaky TWiV 440 TWiV 438 Drs. TWiV go to Washington and the March for Science Unexpected stuff: TWiV 451 Expectorate the unexpected; TWiV 470 Just a passing phage; TWiV 456 Be careful of canons; TWiV 455 Pork and genes; TWiV 432 gamete fusion protein from viruses TWiV 443 On a leaf no one can hear you scream. The puppet master. TWiV 448 Mavis the structure maven Viruses are us: TWiV 472 Riddle of the skinks; TWiV 439 The purloined envelope; TWiV 434 Live long and pupate Antiviral RNA: TWiV 433 Poops viruses and worms; TWiV 449 The sound of non-silencing; TWiV 450 Ben tenOever and RNA out Viruses as tools: TWiV 461 Gotta trace them all Flaviviruses: TWiV 473, 471, 468; 458; 454; 451; 432; 430; 429; 426 NSF survey of earned doctorates Letters read on TWiV 475 Weekly Science Picks Kathy - Voyager recording and book Rich - David Sheldrick Wildlife Trust (Maktao) Dickson - Global Croplands Map Alan - The Great Influenza one day at a time Vincent - The Doomsday Machine by Daniel Ellsberg Listener Picks Mark - Science Moms and Science of HIV Andy - Ask Me Anything Steve - Critical Attitude in Medicine Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiVanguardians take on Bodo saltans virus, a leviathan which infects an abundant flagellated eukaryote in Earth's waters. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu Support Microbe.tv Anti-dengue antibody and disease (J Inf Dis) Bodo saltans virus (biorXiv) Kinetoplast DNA (Euk Cell) Crossword puzzle for book contest (pdf) Letters read on TWiV 474 Weekly Science Picks Kathy - Every second Dickson - Top 10 Science Images 2017 Alan - Shortwave Radiogram Vincent - Science March on Instagram Listener Picks Islam - Class Crossword (Armored Penguin) Peter - The Last of the Iron Lungs Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The Fellowship of the Virus discuss enhancement of dengue disease in humans: the contribution of antibody concentration and increased binding to Fc receptors. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu Support Microbe.tv NEIDL is approved NIH lifts GOF moratorium (Science) Blackburn steps down as head of Salk Institute (SD Union Trib) ADE of dengue disease in humans (Science) Commentary on ADE of dengue disease (Science) ADE of dengue disease and antibody binding to Fc (Science) Letters read on TWiV 473 Weekly Science Picks Kathy - Today in Science History Dickson - 10 Breakthrough Technologies 2017 Rich - SpaceX to orbit a Tesla Alan - Debt in America Vincent - The Death of Expertise by Tom Nichols (NYTimes review) Listener Picks Stephen - A Concise Paper Johnye - Holiday song parodies (one, two, three) Tom - SMBC: Are viruses alive? Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent speaks with professional podcast producer Ray Ortega about his career and the power of the podcast. Host: Vincent Racaniello Guest: Ray Ortega Become a patron of TWiV! Links for this episode Ray on Twitter The Podcasters' Studio Produce Picker Podcast Ray's YouTube channel Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV team reveal the origin of the poxvirus membrane, and how a retrovirus drove the development of the placenta of a lizard. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu Support Microbe.tv Origin of poxvirus membrane (PNAS) Poxvirus movies: Movie S1, Movie S2, Movie S3, Movie S4, Movie S5 Lizard placenta from a retrovirus (PNAS) Letters read on TWiV 472 Weekly Science Picks Kathy - Google doodles Discoverer of photosynthesis Robert Koch Dickson - Nature Photography Winners Rich - The Raging Idiots Planet Song Alan - Zooborns Vincent - Glass Insects and Plants by Yuki Tsunoda Listener Picks Scott - Sagan Series Paul - SMBC Comics Trudy - If Scientists Wrote Christmas Songs Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiVerinos discuss restriction of dengue virus vaccine by Sanofi, and data which suggest that Dengvaxia causes enhanced disease in previously uninfected recipients. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu Sanofi update on Dengvaxia Trouble for dengue vaccine (Reuters) Sanofi restricts dengue vaccine (CIDRAP) Dengvaxia enhances disease (Vaccine) Interview with Jeff Almond Dengue vaccine on TWiV 319, TWiV 370, TWIV 384 Image credit Letters read on TWiV 471 Weekly Science Picks Kathy - NASA is reinventing the wheel Dickson - Stunning neuroscience images Rich - Moneyball by Michael Lewis (film) Alan - Diverse Sources Vincent - Zombie gene protects elephants from cancer (Quanta, TWiEVO) Listener Picks Scott - Why Vaccines Matter by Cynthia Gorney and The Song of the Dodo by David Quammen. Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV ninjas reveal that bacteriophage particles rapidly move across monolayers of eukaryotic cells from different tissues. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode Transcytosis of bacteriophage across cell layers (mBio) Image credit Letters read on TWiV 470 This episode is brought to you by Blue Apron. Blue Apron is the #1 fresh ingredient and recipe delivery service in the country. Get $30 off your first delivery and FREE SHIPPING by going to blueapron.com/twiv. Weekly Science Picks Kathy - Photo of 3200 year-old tree in one image Dickson - Microsculpture Rich - A Short History Of Humans And Germs Alan - Planets app Vincent - Laptops Are Great. But Not During a Lecture or a Meeting Listener Picks Basel - Joint Pathology Center John - OpenStax Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV hosts discuss a plant virus that infects a fungus, and whether you need to work insane hours to succeed in science. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode Phytopathogenic fungus hosts a plant virus (PNAS) Franklin column (PNAS) Potato famine mystery solved (History) Protein Data Bank You do not need to work 80 hours per week (THE) How I Learned To Stop Worrying (Sci Am) Image credit: Truth Wins by Jon Yewdell Letters read on TWiV 469 This episode is brought to you by the Department of Microbiology at the Icahn School of Medicine at Mount Sinai. Composed of over 20 virology labs, all centralized in one building in the heart of New York City, this department is a perfect fit for anyone with an interest in pursuing virus research. For more information about the Department, visit http://bit.ly/micromssm This episode is brought to you by the Virology and Gene Therapy PhD program at the Mayo Clinic Graduate School of Biomedical Sciences in Rochester, Minnesota. The renowned Mayo Clinic Virology and Gene Therapy program is currently accepting applications until December first. If you want to be trained in the exciting fields of oncolytic virotherapy, gene therapy, vaccine creation, or basic virology, visit mayoclinic.org to apply to the Virology and Gene Therapy program at the Mayo Clinic Graduate School of Biomedical Sciences. Weekly Science Picks Kathy - Patterns Video (WonderLab) Dickson - Nikon 2017 Photomicrography Contest Rich - Leonardo Da Vinci by Walter Isaacson Alan - Sail Magazine Best Boats 2018 Vincent - Science Is Dry, Obscure, Complex? Listener Picks Ben - Is the staggeringly profitable business of scientific publishing bad for science? Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Amy joins the TWiV team to talk about her career and her work on Zika virus neurotropism using embryonic mouse organotypic brain slice cultures. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Amy Rosenfeld Become a patron of TWiV! Links for this episode Grad students face tax hike (Nature) House voted to bankrupt grad students (NYTimes) Neurotropism of early and late Zika virus isolates (PNAS) Zika virus blocks the neuron road (virology blog) Letters read on TWiV 468 This episode is brought to you by the Department of Microbiology at the Icahn School of Medicine at Mount Sinai. Composed of over 20 virology labs, all centralized in one building in the heart of New York City, this department is a perfect fit for anyone with an interest in pursuing virus research. For more information about the Department, visit http://bit.ly/micromssm This episode is brought to you by the Virology and Gene Therapy PhD program at the Mayo Clinic Graduate School of Biomedical Sciences in Rochester, Minnesota. The renowned Mayo Clinic Virology and Gene Therapy program is currently accepting applications until December first. If you want to be trained in the exciting fields of oncolytic virotherapy, gene therapy, vaccine creation, or basic virology, visit mayoclinic.org to apply to the Virology and Gene Therapy program at the Mayo Clinic Graduate School of Biomedical Sciences. Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Jon and Teddy Yewdell join the TWiV team to talk about their careers, their research, and the problems with biomedical research. Hosts: Vincent Racaniello, Alan Dove,and Rich Condit Guest: Jon Yewdell and Teddy Yewdell Become a patron of TWiV! Links for this episode Jon Yewdell laboratory Chaudhuri laboratory Truth Wins by Jon Yewdell (epub or mobi download) Noncoding RNAs and class switch recombination (Int Imm) Translating DRiPs (J Leuk Biol) Lamprey VLRP response to influenza virus (eLife) The Biomedical Research Crisis (TWiV 208) This episode is brought to you by the Department of Microbiology at the Icahn School of Medicine at Mount Sinai. Composed of over 20 virology labs, all centralized in one building in the heart of New York City, this department is a perfect fit for anyone with an interest in pursuing virus research. For more information about the Department, visit http://bit.ly/micromssm Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From Indiana University, Vincent and Kathy speak with Tuli Mukhopadhyay, John Patton, and Adam Zlotnick about their careers and their work on alphaviruses, hepatitis B virus, and rotaviruses. Host: Vincent Racaniello and Kathy Spindler Guests: Tuli Mukhopadhyay, John Patton, and Adam Zlotnick Become a patron of TWiV! Links for this episode Mukhopadhyay lab Patton lab Zlotnick lab Tuli on Twitter Palmitoylation of Sindbis virus TF protein (J Virol) Hepatitis B virus structure (Ann Rev Virol) Rotavirus RNA polymerase (Curr Opin Virol) Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Theodora Hatziioannou joins the TWiV team to discuss a macaque model for AIDS, and how a cell protein that blocks HIV-1 infection interacts with double-stranded RNA. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Theodora Hatziioannou Become a patron of TWiV! Links for this episode ASU-UofA Joint Virology Symposium Laboratory of Retrovirology, Rockefeller University HIV-1-induced AIDS in monkeys (Science) APOBEC3H bound to duplex RNA (Nat Comm) Center for HIV RNA studies Image: Two molecules of APOBEC3H bound to dsRNA This episode is brought to you by Blue Apron. Blue Apron is the #1 fresh ingredient and recipe delivery service in the country. Get $30 off your first delivery and FREE SHIPPING by going to blueapron.com/twiv. This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Department of Defense, the Agency's Chemical and Biological Technologies Department hosts the 2017 Chemical and Biological Defense Science & Technology Conference to exchange information on the latest and most dynamic developments for countering chemical and biological weapons of mass destruction. Find out more at http://www.cbdstconference.com Weekly Science Picks Theodora - To Kill A Mockingbird by Harper Lee Kathy - 25 MILLION Orbeez in a pool Dickson - Fluid Dynamics of Paint Rich - The Hitchhiker's Guide to the Galaxy by Douglas Adams Alan - 30 days at sea: timelapse Vincent - TWiEVO 24 and Every Time Zone Listener Picks Fernando - Lego Women of NASA Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

At Tufts University Dental School in Boston, Vincent speaks with Katya Heldwein and Sean Whelan about their careers and their work on herpesvirus structure and replication of vesicular stomatitis virus. Host: Vincent Racaniello Guests: Katya Heldwein and Sean Whelan Become a patron of TWiV! Links for this episode Crystal structure of HSV gB (Science) Crystal structure of HSV fusion regulator gH-gL (Nat Struct Mol Biol) Nuclear Exodus: Herpesviruses Lead the Way (Ann Rev Virol) VSV Pseudotypes Bearing gB, gD, gH, and gL (J Virol) Recovery of infectious VSV from cDNA clones (PNAS) Structure of the L Protein of VSV (Cell) Unique strategy for mRNA cap methylation by VSV (PNAS) Molecular architecture of VSV RNA polymerase (PNAS) This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Department of Defense, the Agency's Chemical and Biological Technologies Department hosts the 2017 Chemical and Biological Defense Science & Technology Conference to exchange information on the latest and most dynamic developments for countering chemical and biological weapons of mass destruction. Find out more at http://www.cbdstconference.com Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiViridae review the 2017 Nobel Prizes for cryoEM and circadian rhythms, and discuss modulation of plant virus replication by RNA methylation. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode Truth Wins by Jonathan Yewdell (epub or mobi) Gabriel Victora awarded MacArthur Prize Forty Years of mRNA Splicing (CSH) 2017 Chemistry Nobel: Jacques Dubochet, Joachim Frank, Richard Henderson 2017 Physiology or Medicine Nobel: Jeffrey C. Hall, Michael Rosbash, Michael W. Young 2017 Nobel Prize in Chemistry (pdf) 2017 Nobel Prize in Physiology of Medicine (pdf) Jonathan Weiner on TWiEVO 19 RNA methylation modulates plant virus infectivity (PNAS) Letters read on TWiV 463 This episode is brought to you by Blue Apron. Blue Apron is the #1 fresh ingredient and recipe delivery service in the country. Get $30 off your first delivery and FREE SHIPPING by going to blueapron.com/twiv. This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Department of Defense, the Agency's Chemical and Biological Technologies Department hosts the 2017 Chemical and Biological Defense Science & Technology Conference to exchange information on the latest and most dynamic developments for countering chemical and biological weapons of mass destruction. Find out more at http://www.cbdstconference.com Weekly Science Picks Kathy - When biospheres collide Dickson - Blame 10 rivers for ocean plastic Alan - Farming in The Netherlands Vincent - Meet the Microbiologist Listener Picks Jay - OmegaTau podcast Trudy - Arrowsmith by Sinclair Lewis Mark - iOS app Human Anatomy Atlas and Master in Business podcast Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

David Tuller returns to discuss his efforts to expose the methodological and ethical problems with the PACE trial for ME/CFS. Hosts: Vincent Racaniello Guest: David Tuller Become a patron of TWiV! Links for this episode All of David's ME/CFS articles at virology blog David Tuller on Twitter Through the Shadowlands by Julie Rehmeyer Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent speaks with 1993 Nobel Laureate Phillip A. Sharp about his career and his seminal discovery of RNA splicing in mammalian cells, which changed our understanding of gene structure. Hosts: Vincent Racaniello Guest: Phillip A. Sharp Become a patron of TWiV! Links for this episode Meet the Microbiologist Sharp Laboratory Spliced segments of adenovirus late mRNA (PNAS) Video of this interview (YouTube) This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Department of Defense, the Agency's Chemical and Biological Technologies Department hosts the 2017 Chemical and Biological Defense Science & Technology Conference to exchange information on the latest and most dynamic developments for countering chemical and biological weapons of mass destruction. Find out more at http://www.cbdstconference.com Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiVers discuss the declining readability of scientific texts, and review the use of self-inactivating rabies virus for tracing neural circuits. Hosts: Vincent Racaniello, Dickson Despommier, Rich Condit, and Kathy Spindler Guest: Brianne Barker Become a patron of TWiV! Links for this episode Meet the Microbiologist TWiV 1: West Nile Virus Decreasing readability of scientific texts (eLife) Measure text readability Tracing neural circuits with self-inactivating rabies virus (Cell) Cre driver network (NIH) Monosynaptic tracing with rabies virus (Neuron) Letters read on TWiV 461 This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Department of Defense, the Agency's Chemical and Biological Technologies Department hosts the 2017 Chemical and Biological Defense Science & Technology Conference to exchange information on the latest and most dynamic developments for countering chemical and biological weapons of mass destruction. Find out more at http://www.cbdstconference.com Weekly Science Picks Kathy -Best eclipse video (to date) and solargraph Dickson - Photos by Will Eades Brianne - Pale Rider by Laura Spinney Rich - "Supergenes" Drive Evolution Vincent -Anatomy of a Moral Panic Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent travels to the University of Pennsylvania and speaks with virologists Gary Cohen, Scott Hensley, Carolina Lopez, and Susan Weiss about their careers and their research. Host: Vincent Racaniello Guests: Gary Cohen, Scott Hensley, Carolina Lopez, and Susan Weiss Become a patron of TWiV! Links for this episode Cohen Laboratory Hensley Laboratory Lopez Laboratory Weiss Laboratory This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Department of Defense, the Agency's Chemical and Biological Technologies Department hosts the 2017 Chemical and Biological Defense Science & Technology Conference to exchange information on the latest and most dynamic developments for countering chemical and biological weapons of mass destruction. Find out more at http://www.cbdstconference.com Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV team reviews the first FDA approved gene therapy, accidental exposure to poliovirus type 2 in a manufacturing plant, and production of a candidate poliovirus vaccine in plants. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASM Conference on Viral Manipulation of Nuclear Processes ASM Public Outreach Fellowship Kymriah approved (PennMed) CAR T cells (NCBI) Cost of Kymriah (NYTimes) Accidental exposure to poliovirus type 2 (Eurosurveill) GAPIII (WHO) Poliovaccine candidate in plants (Nat Commun) Vertical vaccine farm (TWiV 47) All picornaviruses, all the time (TWiV 425) Image credit Letters read on TWiV 459 This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Department of Defense, the Agency's Chemical and Biological Technologies Department hosts the 2017 Chemical and Biological Defense Science & Technology Conference to exchange information on the latest and most dynamic developments for countering chemical and biological weapons of mass destruction. Find out more at http://www.cbdstconference.com Weekly Science Picks Kathy - Cassini photos Dickson - Caliber Biotherapeutics Alan - Grav Rich - Google street view of the International Space Station (article) Vincent - the bioinformatics chat Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiVians present an imported case of yellow fever in New York City, and explain how a dengue virus subgenomic RNA disrupts immunity in mosquito salivary glands to increase virus replication. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASM Conference on Viral Manipulation of Nuclear Processes Roger W. Hendrix, 74 (Pittsburgh Post-Gazette) Roger Hendrix video on TWiV #135 Yellow fever in traveler returning from Peru (MMWR) Dengue subgenomic RNA in mosquito salivary gland (PLoS Path) Image credit Letters read on TWiV 458 This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Department of Defense, the Agency's Chemical and Biological Technologies Department hosts the 2017 Chemical and Biological Defense Science & Technology Conference to exchange information on the latest and most dynamic developments for countering chemical and biological weapons of mass destruction. Find out more at http://www.cbdstconference.com Weekly Science Picks Kathy - Antibody validation Editorial Asilomar meeting report Dickson - Despommierphotoart.com Alan - NOAA Hurricane Hunters Rich - Awakenings by Oliver Sacks (encephalitis lethargica) Vincent - Goodnight Lab by Chris Ferrie Listener Picks Pete - Change Agent by Daniel Suarez Paul - Pasteur's Gambit by Stephen Dando-Collins Maureen - Discovery Documentary First in Human Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Brianne returns to the TWiV Gang to discuss the distribution of proteins on the influenza viral genome, and the evolution of myxoma virus that was released in Australia to control the rabbit population. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Rich Condit Guest: Brianne Barker Become a patron of TWiV! Links for this episode First genetically engineered salmon sold in Canada Nucleoprotein on influenza virus RNA (Nucl Acids Res) Evolution of released myxoma virus in Australia (PNAS) Image credit Letters read on TWiV 457 This episode is brought to you by Blue Apron. Blue Apron is the #1 fresh ingredient and recipe delivery service in the country. See what's on the menu this week and get your first 3 meals free with your first purchase - WITH FREE SHIPPING - by going to blueapron.com/twiv. This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Department of Defense, the Agency's Chemical and Biological Technologies Department hosts the 2017 Chemical and Biological Defense Science & Technology Conference to exchange information on the latest and most dynamic developments for countering chemical and biological weapons of mass destruction. Find out more at http://www.cbdstconference.com Weekly Science Picks Brianne - 3D printed viruses Dickson - Secrets of Schooling Alan - Making sails in the Nevada desert Rich - David Vetter isolation suit (Wiki) Vincent - Confounds the Science Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Brianne joins the TWiVMasters to explain how mutations in genes encoding RNA polymerase III predispose children to severe varicella, and detection of an RNA virus by a DNA sensor. Hosts: Vincent Racaniello and Rich Condit Guest: Brianne Barker Become a patron of TWiV! Links for this episode RNA pol III mutations underlie severe varicella (J Clin Inves) Dengue virus activates cGAS by release of mitochondrial DNA (Sci Rep) Dengue virus NS2B protein targets cGAS for degradation (Nat Micr) Image credit Letters read on TWiV 456 This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Department of Defense, the Agency's Chemical and Biological Technologies Department hosts the 2017 Chemical and Biological Defense Science & Technology Conference to exchange information on the latest and most dynamic developments for countering chemical and biological weapons of mass destruction. Find out more at http://www.cbdstconference.com Weekly Science Picks Brianne - Up-Goer Five Challenge (related to Thing Explainer) Rich - The Farthest: Voyager in Space and Vipassana Momma: Science Vincent - Episode of Revisionist History: The Basement Tapes Listener Pick Jennie - What Piece of Lab Equipment Are You? Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Erin joins the TWiVirions to discuss a computer exploit encoded in DNA, creation of pigs free of endogenous retroviruses, and mutations in the gene encoding an innate sensor of RNA in children with severe viral respiratory disease. Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler Guest: Erin Garcia Become a patron of TWiV! Links for this episode DNA-based computer exploit (pdf) Inactivation of porcine endogenous retrovirus in pigs (Science) Severe viral respiratory infections in children with IFIH1 mutations (PNAS) IRF7 deficiency and severe respiratory infection (TWiV 336) Image credit Letters read on TWiV 455 This episode is brought to you by the Defense Threat Reduction Agency. Part of the U.S. Department of Defense, the Agency's Chemical and Biological Technologies Department hosts the 2017 Chemical and Biological Defense Science & Technology Conference to exchange information on the latest and most dynamic developments for countering chemical and biological weapons of mass destruction. Find out more at http://www.cbdstconference.com Weekly Science Picks Erin - What If? by Randall Monroe Rich - Art.Science.Gallery (J. Haley Gillespie) Kathy - Astronomy photographer of the year shortlist Alan - Upcoming Eclipses Vincent - Ford Thunderbird Automatic Transmission Rebuild Listener Pick Bryan - Neuroscientists tricks journals Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Sharon and Scott return to TWiV for a Zika virus update, including their work on viral evolution and spread, and whether pre-existing immunity to dengue virus enhances pathogenesis. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Guests: Sharon Isern and Scott Michael Become a patron of TWiV! Links for this episode Hurricane Zika (TWiV 410) 454 Corporation (Wikipedia) Regional Zika update, Americas (PAHO, WHO) Zika virus serum bank (pdf) Zika virus evolution and spread in the Americas (Nature) Multiple introductions of Zika virus in US (Nature) Inhibition of Zika Virus Replication by Wolbachia (J Virol) Zika virus pathogenesis in rhesus macaques unaffected by immunity to dengue virus (Nat Commun) Impact of flavivirus immunity on Zika virus infection in rhesus macaques (PLoS Path) Enhancement of Zika virus pathogenesis by antiflavivirus immunity (Science) No ADE by dengue antibodies in Zika virus patients (Clin Inf Dis) Oropharyngeal mucosal transmission of Zika virus in rhesus macaques (Nat Commun) Letters read on TWiV 454 Weekly Science Picks Dickson - Industrial Scars Kathy - Eclipse info by zipcode Sharon - Michaela's article in The Conversation Scott - Signal P, Pecaan, Phamerator, DNAmasterAlan - The Eagle Whisperer" (and the Great Big Story channel) Vincent - Bottle Listener Pick Kevin - Tenure track scientist bites the dust Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From the Vector-Borne Viruses Symposium in Hamilton, Montana, Dickson and Vincent speak with Diane Griffin about her career and her work on understanding viral infections of the central nervous system. Hosts: Vincent Racaniello and Dickson Despommier Guest: Diane Griffin Become a patron of TWiV! Links for this episode Vector-Borne Viruses Symposium Diane Griffin Adoptive immunization of immunosuppressed mice infected with Sindbis virus Role of the immune response in age-dependent resistance of mice to encephalitis due to Sindbis virus Antibody-mediated clearance of alphavirus infection from neurons Persistence of viral RNA in mouse brains after recovery from acute alphavirus encephalitis Conversion of lytic to persistent alphavirus infection by the bcl-2 cellular oncogene Bc1-2 protects mice against fatal alphavirus encephalitis This episode is brought to you by Blue Apron. Blue Apron is the #1 fresh ingredient and recipe delivery service in the country. See what's on the menu this week and get 3 meals free with your first purchase – WITH FREE SHIPPING – by going to blueapron.com/twiv. Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Lynda Coughlan joins the weekly virtual bus companions for a discussion of a host defense peptide from frogs that destroys influenza virus, and mouse models for acute and chronic hepacivirus infection. Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler Guest: Lynda Coughlan Become a patron of TWiV! Links for this episode Linda on Virus Watch and BSGCT Anti-influenza peptide from frogs (Immunity) Magainins (Wikipedia) Urumi Kathy's defensin paper (PLoS Path) World Hepatitis Day Mouse model for hepacivirus infection (Science) Letters read on TWiV 452 Weekly Science Picks Kathy - US rivers as subway map Lynda - No Time to Lose: A Life in Pursuit of Deadly Viruses by Peter Piot Alan - Apollo-era computers found in dead engineer's basement Vincent - 110 NFL Brains, research article in JAMA and The best science is often accidental Listener Pick Stig - Security Now! and The Gene by Siddhartha Mukherjee Ricardo - John Oliver on Vaccines Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV scientists reveal that mosquitoes transmit different West Nile virus populations with each blood meal, only to have the diversity purged in a bird host. Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode Driving past a milestone (TWiP 100) Debug Fresno (Verily blog) What could possibly go wrong? (TWiV 388) Chestnut blight (ProMedMail) Hypovirus molecular biology (Adv Virus Res) Mosquitoes transmit unique WNV populations (Cell Rep) Teaching science using pseudoscience Image credit Letters read on TWiV 451 Weekly Science Picks Kathy - Approaching Jupiter and Michigan parents about vaccination Rich - Math Limericks from Futility Closet Alan - NASA flyover of Pluto Vincent - Talk show host nominated as USDA chief scientist Listener Pick Neil - These smart girls are here to debunk anti-vaxxer nonsense John - John Sever AMA Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Ben tenOever joins the TWiVoli to discuss the evolution of RNA interference and his lab's finding that RNAse III nucleases, needed for the maturation of cellular RNAs, are an ancient antiviral RNA recognition platform in all domains of life. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Ben tenOever Become a patron of TWiV! Links for this episode RNAseIII is an ancient antiviral effector (Nature) Influenza suppression of RNAi (PNAS) Questioning mammalian antiviral RNAi (Nat Micro) Antiviral protection via RdRp (PLoS Path) Evolution of antiviral defense (Cell Host Micr) Letters read on TWiV 450 Weekly Science Picks Ben - Invisible Invaders by Peter Radetsky Kathy - ASV 2017 Virolympics Crossword solved (pdf) and National Museum of the Air Force Rich - Columbia river gorge and Undaunted Courage by Stephen Ambrose Dickson - All the World's Earthquakes Alan - Map of Roman roads Vincent - Brain atlas of fly behavior Listener Pick Rob - Vaccinate Your Kids and Charles Darwin Natural Selection Islam - Self-assembling virus and Virus Patterns Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV Council explores the finding that facial appearance affects science communication, and evidence that RNA interference confers antiviral immunity in mammalian cells. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Become a patron of TWiV! Links for this episode JMBE SciComm Issue Facial appearance affects science communication (PNAS) Your face matters to science (virology blog) RNA based antiviral immunity in mammals (Immunity) How mice say nodavirus (TWiV 245) Press release on science meeting gender disparity The Traditional Lecture is Not Dead. I Would Know – I'm A Professor (virology blog) Sound of Silence by Disturbed (YouTube) Letters read on TWiV 449 Weekly Science Picks Kathy - ASV 2017 Virolympics Crossword (pdf) Dickson - The Visible Mouse Alan - Is profitable publishing bad for science? Vincent - Locally Sourced Science Listener Pick Neva - Michael Summers interview and Virus coloring book Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From ASV 2017 in Madison, Wisconsin, the complete TWiV team speaks with Mavis Agbandje-McKenna about her career and her work solving virus structures by x-ray crystallography and cryo-electron microscopy. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Mavis Agbandje-McKenna Become a patron of TWiV! This episode of TWiV is sponsored by Promega Corporation. Promega is a leader in providing innovative solutions and technical support to the life science industry. The company's 3,500 products support scientists' worldwide to advance their knowledge in genomics, proteomics, cellular analysis, drug discovery and human identification. Founded in 1978, the company is headquartered in Madison, WI with branches in 16 countries and over 50 global distributors. Promega products are used by life scientists who are asking fundamental questions about biological processes applying their knowledge to diagnose and treat diseases or discover new therapeutics. For more information about Promega, visit www.promega.com/ASV Weekly Science Picks Kathy - Doktor Kaboom! Dickson - Mars as you've never seen it before Alan - Eclipse 2017 Rich - Sirens of Titan by Kurt Vonnegut Vincent - Research!America Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The glorious TWiVerati un-impact their email backlog, anwering questions about viruses, viruses, viruses, viruses, viruses, and more. You should listen - our fans ask great questions! Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode JMBE SciComm Issue Female role models in engineering (PNAS) Gender equity at ASM meeting (mBio) Invisible Frontiers Time, Love, Memory The Eighth Day of Creation Predatory journals recruit fake editor Photo by Anthony Olszewski Letters read on TWiV 447 Weekly Science Picks Kathy - Oskar Fischinger Google Doodle Dickson - Ten Tiny But Terrifying Micro Monsters Alan - Hunting mammoth ivory in Siberia Rich - Occam's razor Vincent - Blame vax for illness without proof, says EU Listener Pick Ed - Lysenkoism Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV hosts review an analysis of gender parity trends at virology conferences, and the origin and unusual pathogenesis of the 1918 pandemic H1N1 influenza virus. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASM grant writing online course Gender parity trends at virology meetings (J Virol) Professor gender perpetuates gender gap (NBER) Next generation researchers initiative (NIH) NIH abandons grant cap (Science) Genesis and pathogenesis of 1918 H1N1 influenza virus (PNAS) Image credit Letters read on TWiV 446 Weekly Science Picks Kathy - BioKids Critter Catalog Dickson - Shanghai Vertical Farm Alan - Colossal Cave Adventure open source Rich - Life on Mars (HI-SEAS) Vincent - TWiM 154 with Arturo Casadevall Listener Pick Grant - Antibody validation challenges Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From Nido2017 in Kansas City, Vincent meets up with three virologists to talk about their careers and their work on nidoviruses. Host: Vincent Racaniello Guests: Margo Brinton, Luis Enjuanes, and Linda Saif Become a patron of TWiV! Links for this episode Murine flavivirus resistance gene (PNAS) TIA-1/TIAR binding sites in West Nile virus RNA (J Virol) Infectious coronavirus DNA in BAC (PNAS) Norovirus binding to lettuce (Appl Env Micro) This episode is brought to you by Blue Apron. Blue Apron is the #1 fresh ingredient and recipe delivery service in the country. See what's on the menu this week and get your first 3 meals free with your first purchase – WITH FREE SHIPPING – by going to blueapron.com/twiv Intro music is by Ronald Jenkees Send your virology questions and comments to [email protected]

From ASM Microbe 2017 at New Orleans, Vincent and Rich meet up with astronaut Kate Rubins to talk about becoming an astronaut, space travel, and doing science in space. Hosts: Vincent Racaniello and Rich Condit Guest: Kate Rubins Become a patron of TWiV! Links for this episode Live from the Space Station, NASA Astronaut Kate Rubins Kate Rubins (NASA, Wikipedia) Expedition 48-49 launches to the ISS Expedition 48-49 Crew Docks to the Space Station Expedition 48-49 Crew Welcomed to the Space Station Expedition 48 Crew Hands Over the Space Station to Expedition 49 Photo by Chris Condayan Intro music is by Ronald Jenkees Send your virology questions and comments to [email protected]