This Week in Virology

The TWiV team returns this week to SARS-CoV-2019 coverage to review the latest epi curves, the fatality rate, furin cleavage site and receptor binding domain in the spike glycoprotein, related CoV recovered from pangolins, evidence that the virus did not escape from a laboratory, and many more questions sent in by listeners. Hosts: Vincent Racaniello, Dickson Despommier, Rich Condit, and Kathy Spindler Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode WHO CoV sitreps Epidemiology of COVID-19 (China CDC) COVID-19 incubation period (J Med Virol) China clinical trials for COVID-19 (Nature) Furin cleavage site in SARS-CoV-2 spike (virology blog) Proximal origin of SARS-CoV-2 (Virological) Pangolin photos (Nat Geo) Letters read on TWiV 588 Timestamps by Jolene. Thanks! Weekly Science Picks Kathy - The deep sea Rich - World's oldest message in a bottle Dickson - Coronavirus: A visual guide Vincent - Recent NSABB meeting Videocasts Day 1 Day 2 Listener Picks Stephen - This podcast will kill you Neva - China's surveillance Sophia - WHO online courses Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent and Rich return to the University of Texas Medical Branch at Galveston to speak with Dennis Bente, Shannan Rossi, Nikos Vasilakis, and Scott Weaver about their work on viruses transmitted by mosquitoes and ticks. Hosts: Vincent Racaniello and Rich Condit Guests: Dennis Bente, Shannan Rossi, Nikos Vasilakis, and Scott Weaver Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Measles virus vectored chikungunya vaccine (J Inf Dis) World Reference Center for Emerging Viruses and Arboviruses Insect-specific alphavirus vaccine vector (Nat Med) Legacy of arbovirus hunters (Viruses) Electron microscopy for novel arboviruses (Viruses) Sylvatic dengue (Am J Trop Med Hyg) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

In this week's coverage of the coronavirus epidemic, the TWiV team discusses the fatality rate, China's initial reaction to the outbreak, conspiracy theories, how long the virus remains infectious on surfaces, and evidence for virus in the intestinal tract. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Brianne Download TWiV 586 (72 MB .mp3, 119 min) Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode China reacted too slowly to epidemic (NY Times) Wuhan rounds up infected (NY Times) 2019-nCoV asymptomatic transmission report flawed (Science) Mucus reduces effectiveness of alcohol gels (mSphere) SARS-CoV in feces (NEJM, Gastroenterology) Inadequate plumbing and spread of SARS-CoV (WHO) Persistence of virus on surfaces (one, two, three, four) Letters read on TWiV 586 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - Mutation, Adaptation, and Virus Genomes Alan - The Comet Rich - Finding Your Roots: Science Pioneers Dickson - Let's Fill Our Cities With Taller, Wooden Buildings Vincent - Twenty-first century plague by Thomas Abraham Listener Pick Mark - Tracking CoV infections Sophia - Legislative approaches to immunization Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiVerers continue their coverage of the new coronavirus outbreak in China, as the number of cases increase dramatically and the virus begins person-to-person transmission in other countries. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, Kathy Spindler, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode ASV 2020 Real time tracking of 2019-nCoV (JHU) Estimation of 2019-nCoV R0 (Int J Inf Dis) WHO Sitreps on 2019-nCoV Coronavirus overtakes SARS (SCMP) HHS press conference Discovery of 2019-nCoV (bioRxiv) Rich CoV genes in bats (PLoS Path) Early 2019-nCoV transmission dynamics (NEJM) Clinical features of early 2019-nCoV patients (Lancet) Remdesvir for MERS-CoV (Nat Commun) Transmissibility of 2019-nCoV (Imperial Coll, pdf) Comparison of R0 of pathogens (Guardian) Forecast of 2019-nCoV (WHO, Univ Hong Kong, pdf) Quammen on 2019-nCoV (NY Times) Benefits of handwashing (NY Times) Asymptomatic 2019-CoV transmission (NEJM) Summary of TWiV 584 Surgical vs N95 masks (JAMA) Letters read on TWiV 585 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - Pandemic Alan - Educated by Tara Westover Rich - Star Trek: Picard Dickson - Highest Resolution Image Ever Taken of Surface of the Sun Kathy - Scientists make vampire bats 'glow' to simulate vaccine spread Vincent - Virology Lectures 2020 Listener Pick Edward - Virus snowflake designs Anthony - Microbe Hunting Autism to Zika Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Coronavirus expert Ralph Baric joins TWiV to explain the virology and epidemiology of the recent zoonotic outbreak spreading across China and overseas. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove,Rich Condit, Kathy Spindler Guest: Ralph Baric Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode ASV 2020 WHO coronavirus sitreps CIDRAP article on coronavirus vaccines Phylogenetic analysis of 2019-nCoV ACE2 is 2019-nCoV receptor (bioRxiv) Visual explainer of Huanan market (SCMP) Clinical features of 2019-nCoV patients (Lancet) Familial cluster of pneumonia caused by 2019-nCoV (Lancet) SARS-CoV 29 nt deletion reduces replication (Sci Rep) NSABB 2019 TWiV World Tour t-shirt Letters read on TWiV 584 Timestamps by Jolene. Thanks! Weekly Science Picks Alan - Library plug-in for Firefox or Chrome Rich - Sounds of the Solar Wind (Parker Solar Probe) Dickson - Stunning wildlife photos Kathy - Incomplete list of things that blow the minds of intro bio students Vincent - Mosquito photographs by Alex Wild (and anti-pick) Listener Pick Justin - YadaYadaYada virus Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent and Rich travel to Galveston National Laboratory to speak with Jim LeDuc, Tom Ksiazek, and Bob Tesch about their long careers as virus hunters. Hosts: Vincent Racaniello and Rich Condit Guests: Jim LeDuc, Tom Ksiazek, and Bob Tesh Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

TWiV provides updates on the new coronavirus causing respiratory disease in China, the current influenza season, and the epidemic of African swine fever, including determination of the three-dimensional structure of the virus particle. Hosts: Vincent Racaniello, Dickson Despommier, Rich Condit, Kathy Spindler, and Brianne Barker Download TWiV 582 (71 MB .mp3, 118 min) Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode ASV 2020 NJ ready to remove religious vaccine exemptions (Patch) New virus causing pneumonia in China (NY Times) New China virus is coronavirus (NY Times, ProMedMail) Weekly influenza cases (CDC) Future of influenza vaccines (Vaccines) Household influenza vaccine study (Int J Epidemiol) African swine fever (USDA) African swine fever infographics (one, two) Race for African swine fever vaccine (Scientist) 3D structure of African swine fever virus (J Biol Chem) Letters read on TWiV 582 Timestamps by Jolene. Thanks! Weekly Science Picks Rich - Mayo Clinic - Ken Burns; Patient Care and Health Information Dickson - Australia Fires; Insect ecology; Smoke and aerosols Kathy - runcible Vincent - Without a Vaccine, These Are Your Odds Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

For the first TWiV of 2020 we reveal that microbiome depletion with antibiotics alters the immune response to influenza vaccine, and how successive blood meals facilitate virus dissemination in mosquitoes and transmission potential. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Kathy Spindler, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode ASV 2020 Vaccines are safe and effective (Science) Microbiome perturbation alters response to flu vaccine (Cell) Successive blood meals aid virus dissemination in mosquitoes (Nat Micro) Letters read on TWiV 581 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - Retrovirus cookie cutter! (and Phage, Adenovirus, Rabies virus, and Antibody Alan - "Yah! Yah! Yah!" by Jack London, and the true story it's based on Dickson - Why whales are big but not bigger Kathy - Dual Axis Illusion Vincent - The Fall of the Faculty by Benjamin Ginsberg Listener Pick Suellen - Patient Zero podcast Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From the University of Texas at Austin, Vincent and Rich speak with Chris Sullivan about his work on miRNAs encoded in the genomes of polyomaviruses and papillomaviruses, and how an RNA triphosphatase restricts hepatitis C virus replication. Hosts: Vincent Racaniello and Rich Condit Guest: Chris Sullivan Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Sullivan lab Virus-encoded miRNAs in papillomaviruses (PLoS Path) Murine polyomavirus miRNA promotes viruria (J Virol) DUSP11 helps XRN restrict hepatitis C virus (PNAS) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent speaks with virologists at the University of Nevada at Reno about their careers and their work on herpesviruses, arboviruses, and the development of diagnostics for infectious diseases. Host: Vincent Racaniello Guests: Dave AuCoin, Cyprian Rossetto, Claudia Rückert, and Subhash Verma Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode PAN's labyrinth (Viruses) KSHV SOX protein DNAse activity (J Virol) Vertical flow immunoassay for pathogen detection (Talanta) Small RNA responses of mosquitoes (Insect Biochem Mol Biol) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent speaks with members of Prometheus, a team of academic and industrial scientists assembled to develop antibody-based therapeutics against infections caused by tick-borne Crimean-Congo hemorrhagic fever virus and rodent-borne hantaviruses, for which no approved vaccines or specific drugs are available. Host: Vincent Racaniello Guests: Kartik Chandran, Zachary Bornholdt, Steven Bradfute, John Dye, Matthias Forsell, Jason McLellan, Laura Walker, and Cecelia Vial. Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode NIH U19 grant mechanism $22 million Prometheus grant Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent speaks with Félix Rey about his career and his work on solving structures of a variety of viruses and the insights learned about viral membrane fusion and antibody-mediated neutralization. Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Unité de Virologie Structurale Tick-borne encephalitis glycoprotein structure (Nature) HCV RNA polymerase structure (PNAS) Flavivirus structural heterogeneity (Curr Op Viral) Antibody responses to flaviviruses (EMBO Rep) Covalently linked dengue envelope dimers (Nat Comm) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From the 22nd meeting of the European Society for Clinical Virology in Copenhagen, Vincent speaks with Thea, Heli, Kim, Caroline and Irma about big data and its increasing use in virology diagnostics, epidemiology, and public health. Host: Vincent Racaniello Guests: Thea Kølsen Fischer, Kim Benschop, Caroline Brown, Heli Harvala, and Irma Salimovic-Besic Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode 22nd ESCV meeting Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From the Fourth Symposium on Giant Virus Biology in Germany, Vincent, Rich, and Nels speak with Assaf, Stephen, and Alexandra about their careers and their work on giant viruses that infect ocean hosts: Emiliana huxleyi, Aureococcus anophagefferans, and a choanoflagellate. Hosts: Vincent Racaniello, Rich Condit, and Nels Elde Guests: Assaf Vardi, Stephen Wilhelm, and Alexandra Worden Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode 4th Ringberg Symposium on Giant Virus Biology In-plaque mass spectrometry imaging (Nature Micro) Infection by brown tide virus, AaV (Front Micro) Choano virus with a rhodopsin photosystem (PNAS) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From Georgia State, Vincent speaks with economics professor Paula Stephan about the ways science is supported in the US, how universities offload risks, the absence of risk-taking, and much more. Hosts: Vincent Racaniello Guest: Paula Stephan Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode How Economics Shapes Science Coalition for Next Generation Life Science Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

William Summers joins the TWiV team to discuss some virology history, including the ever-changing concept of 'virus' and the contribution of phage research to the study of animal viruses. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Rich Condit Guest: William Summers Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Bill's ASV 2019 talk (PPT download) Inventing viruses (Ann Rev Virol) Timestamps by Jolene. Thanks! Weekly Science Picks Alan - Restoration of Western Flyer Rich - Purification of phage T7 RNA polymerase Dickson - The Galaxy is Not Flat Bill - Efficacy of tetravalent dengue vaccine Vincent - Blowout by Rachel Maddow Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Amy joins the TWiV team to review evidence that enterovirus D68 is an etiologic agent of childhood paralysis, and her finding that the ability of the virus to infect cells of the nervous system is not a recently acquired property. Hosts: Vincent Racaniello, Dickson Despommier, Rich Condit, and Kathy Spindler Guest: Amy Rosenfeld Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode EV-D68 antibodies in humans before 2014 outbreak (Emerg Inf Dis) Enterovirus antibodies in CSF of AFM patients (mBio) Serology implicates enteroviruses in AFM (Nat Med) EV-D68 neurotropism is not new (mBio) Letters read on TWiV 572 Timestamps by Jolene. Thanks! Weekly Science Picks Amy - Enterovirus biology and pathogenesis and Acute Flaccid Myelitis Association Rich - Tesla Dickson - Nikon Small World 2019 Photo Competition Kathy - Term limits in academic leadership #1 and #2 Vincent - Coywolf: New hybrid carnivore Listener Picks Anonymous - Spill the Beans vaccinated shirts Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The League of Extraordinary Virologists celebrate the eradication of wild poliovirus type 3, and consider the effectiveness of an influenza vaccine produced in insect cells, and how small RNAs are protecting the Koala germline from retroviral invasion. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Kathy Spindler, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Flublok (CDC) Adjuvant study of flu vaccines (NIH) Immunogenicity of quadrivalent Flublok (J Inf Dis) Monoclonal antibody response to Flublok (J Virol) Current flu vaccine recommendations (CDC) Double dose flu vaccine immunogenicity (Hum Vac Immunother) piRNA response on retroviral invasion of Koala genome (Cell) piRNAs rise to rescue Koalas (Cell) Image credit Letters read on TWiV 571 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - The Machinery of Life by David Goodsell Alan - Pomological watercolors Dickson - Wildlife Photographer of the Year Kathy - DiversifyMicrobiology DiversifyImmunology and Marmot becomes fox food Vincent - Burnet A Life by Christopher Sexton Listener Picks Justin - Worm with three sexes Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

At Aarhus University in Denmark, Vincent speaks with Trine Mogensen, Søren Paludan, Ole Søgaard, and Madalina Carter-Timofte about their careers and their work on sensing herpesviral DNA, immunodeficiencies that predispose to severe viral infections, and the path to a cure for HIV/AIDS. Hosts: Vincent Racaniello Guests: Trine Mogensen, Søren Paludan, Ole Søgaard, and Madalina Carter-Timofte Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Aarhus University Findaphd.com Sensing incoming HSV-1 (J Int Cyto Res) Be careful of canons (TWiV 456) Polio and host genetics (Front Micro) TLR9 treatment of AIDS (AIDS) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV pro-vaxxers reveal viruses that infect endangered wild salmon, and how iron in host serum modulates dengue virus acquisition by mosquitoes. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode New viruses that infect endangered wild salmon (eLife) Host serum iron modulates dengue virus acquisition by mosquitoes (Nat Micro) Image credit Letters read on TWiV 569 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - Retraction of new CCR5/lifespan study Alan - Nomad Press illustrated science books, and illustrator Lex Cornell Dickson - RARE: Creatures of the Photo Ark Vincent - The Odyssey of Eradication by Thomas Abraham Listener Picks Justin - Mass spec pen Mark - What really brought down the Boeing 737 Max? Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

In the second episode from the Karolinska Institute in Stockholm, Vincent speaks with Jan Albert, Petter Brodin, and Anna Smed-Sörensen about their work on enterovirus D68, systems immunology, and human pulmonary viral infection and inflammation. Hosts: Vincent Racaniello Guests: Jan Albert, Petter Brodin, and Anna Smed-Sörensen Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Karolinska Huddinge Evolution of enterovirus D68 (Virus Evol) Maternal anti-viral antibodies in newborns (Nat Med) Respiratory phagocytes in human influenza (Front Immunol) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV team covers outbreaks of eastern equine encephalitis virus in the US and poliovirus in the Philippines, and explain how a chemokine induced by HIV-1 infection helps release more virus particles from cells. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, Kathy Spindler, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Poliomyelitis returns to the Philippines (Mashable, virology blog) EEE in the USA (Am J Trop Med Hyg) Chemokine helps HIV-1 release (eLIFE) Image credit Letters read on TWiV 567 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - History of infectious disease before/after vaccines Alan - NIH Ham Radio Club Rich - Renewable jet fuel from air Dickson - Newly Discovered Comet Is Likely Interstellar Visitor Kathy - How professors spend their time Vincent - Non-polio enterovirus surveillance network Listener Picks Alexey - Periodic Table Podcasts and Videos Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV team reveals the protein corona that surrounds virus particles and influences infectivity and amyloid aggregation, and a proofreading-impaired herpesvirus that produces quasispecies-like populations. Hosts: Vincent Racaniello, Alan Dove, and Rich Condit Guest: Kiki Warren Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Human fetal tissue research restrictions (The Scientist) Viral protein corona (Nat Commun) Proofreading-impaired herpesvirus (Nat Micro) Phage quasispecies (Cell) Letters read on TWiV 566 Timestamps by Jolene. Thanks! Weekly Science Picks Alan - Astronomy photographer of the year Rich- Urban living leads to high cholesterol... in crows Kiki- Reversal of epigenetic aging Vincent- General Virology by SE Luria Listener Picks Sindhu - Sweden Solar System Anthony- Surprising role of viruses in evolution with Eugene Koonin Paul- The Obscure Virus Club Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From the Karolinska Institute in Stockholm, Vincent speaks with Niklas Björkström, Ali Mirazimi, and Matti Sällberg about their work on the impact of chronic hepatitis C virus infection on NK cells, Crimean-Congo hemorrhagic fever virus vaccines, and immunotherapy to block entry of hepatitis B and D viruses. Hosts: Vincent Racaniello Guests: Niklas Björkström, Ali Mirazimi, and Matti Sällberg Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Karolinska Huddinge CCHV experimental vaccines (J Virol) Increased NK function after antiviral cessation (J Inf Dis) Impact of HCV infection on NK cell repertoire (Nat Comm) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From the 16th Smögen Summer Symposium on Virology, Vincent speaks with Erling Norrby about how he has used archival material to provide insight into early Nobel Prizes for research on viruses. Hosts: Vincent Racaniello Guest: Erling Norrby Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Swedish Society for Virology Nobel Prizes and Life Sciences by Erling Norrby Nobel Prizes and Nature's Surprises by Erling Norrby Nobel Prizes and Notable Discoveries by Erling Norrby Nobel Prizes: Cancer, Vision and the Genetic Code by Erling Norrby Polio and Nobel Prizes (Ann Neurol) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

David Tuller returns to talk about his efforts of the past year to expose the methodological and ethical problems with the PACE and Lightning trials for therapy of ME/CFS. Host: Vincent Racaniello Guest: David Tuller Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiVologists consider whether to receive an influenza vaccine in August (in the northern hemisphere), and mice implanted with human lung fragments for studying microbial pathogens. Hosts: Vincent Racaniello, Alan Dove, Kathy Spindler, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Waning of influenza vaccine protection (Clin Inf Dis) Precision mouse models for microbial pathogenesis (Nat Biotech) Letters read on TWiV 563 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - Why we need to increase diversity in the immunology research community Alan - Stock photos of vaccination that don't suck Kathy- Sample size calculator Vincent- How Economics Shapes Science by Paula Stephan Listener Picks Melinda - Zome Tools Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

TWiV travels to Rutgers University to speak with Brad, Kay, Siobain, and Kim about their careers and their work on viruses of plants, fungi, bacteria, diatoms, and coccolithophores. Hosts: Vincent Racaniello, Dickson Despommier, and Brianne Barker Guests: Brad Hillman, Kay Bidle, Siobain Duffy, and Kim Thamatrakoln Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Viruses of plant-interacting fungi (Adv Virus Res) Why are RNA virus mutation rates so damn high? (PLoS Biol) CRESS viruses (Adv Virus Res) Coccolithovirus facilitation of carbon export in North Atlantic (Nat Micro) Silicon limitation facilitates virus infection of marine diatoms (Nat Micro) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The Autonomous CollecTWiVe reveal two effective treatments for Ebolavirus infection, how a virus in a fungus confers heat tolerance to a plant, and dampened inflammation as a mechanism for bat tolerance to viral infection. Hosts: Vincent Racaniello, Alan Dove, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Two new and effective Ebolavirus treatments (NIH, Nature) A virus in a fungus in a plant (Science) Dampened NLRP3-mediated inflammation in bats (Nat Micro) Bat tolerance to virus infection (Nat Micro) Image credit Letters read on TWiV 561 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - Deaf student makes sign language terms to communicate science Alan - Show Your Stripes Vincent- Sixty Years of Discovery Listener Picks Eric - This drug could end HIV - why hasn't it? Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From the meeting of the Centers of Excellence for Influenza Research and Surveillance, Vincent speaks with Alan, Florian and Jennifer about their careers, the purpose of CEIRS, universal influenza vaccines, and cellular responses to infection in pediatric populations. Host: Vincent Racaniello Guests: Alan Embry, Florian Krammer, and Jennifer Nayak Become a patron of TWiV! Links for this episode NIAID CEIRS 12th CEIRS meeting summary (pdf) Antibody response to influenza virus (Nat Rev Micro) Influenza virus CD4 T cells in children and young adults (Sci Rep) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The complete TWiV team give a report on the Ebola virus outbreak in DRC, and reveal that cell surface nectin proteins cause the transfer of cytoplasmic cargo, including measles virus, between cells. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, Kathy Spindler, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Fire Alarm at ASV Ebola outbreak sitrep(WHO) Ebola outbreak dashboard(WHO) Ebola outbreakDRC (MSF) Ebola articles at CIDRAP Ebola virus vaccine could run out(STAT news) Nectin mediated cytoplasmic transfer(J Cell Sci) Letters readon TWiV 559 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - DukeMed Distinguished Award: Bill Joklik Alan - Audubon photography awards for 2019 Rich - Joklik paper on vaccinia virus mRNA; Joklik MEM Dickson- Ghost orchid pollination revealed Kathy- Crocheted petri plates Vincent- Documents relating to history of ASV Listener Picks Neva - What happens if we engineer a superbug? Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From ASV 2019 at the University of Minnesota, TWiV explores the origins of the American Society for Virology with Sid Grossberg and Pat Spear. Hosts: Vincent Racaniello, Rich Condit, Kathy Spindler, and Brianne Barker Guests: Sid Grossberg and Pat Spear Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode American Society for Virology Bill Joklik, 92 How ASV was founded (Virology) Signatures at the Chicago meeting (page one, page two) Timeline history of ASV (jpg) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From the European Congress of Virology in Rotterdam, Vincent and local co-host Ben Berkhout speak with Ron Fouchier, Rosina Girones, and Marie-Paule Kieny about their careers and their work on influenza virus, environmental virology, and developing an Ebola virus vaccine during an epidemic. Hosts: Vincent Racanielloand Ben Berkhout Guests: Ron Fouchier, Rosina Girones, and Marie-Paule Kieny Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode ECV2019 Drift of human H2N2 viruses (J Viral) Viruses in urban sewage (Sci Tot Environ) Ebola virus vaccine efficacy (Lancet) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

TWiV minus one reveals delayed neurological deficits in children without microcephaly born to Zika virus infected mothers, and N-glycolyl-neuraminic acid as a receptor for influenza A viruses. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Kathy Spindler, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Deficits in children born to Zika virus infected mothers (Nat Med) Zika virus is still a threat (NY Times) N-glycolyl-neuraminic acid as influenza virus receptor (Cell Rep) Letters read on TWiV 556 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - Foldable viral capsid models and Foldable models demonstrate T numbers Alan - Laws Guide to Nature Drawing and Journaling Dickson- NASA 50th Events Kathy- Alta Obscura's 17,069 strange and wonderful places Vincent- TWiEVO 44: The enemy of my enemy is my phage Listener Picks Anthony - Global Water Pathogen Project Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

From ASM Microbe 2019, Vincent, Brianne and Calvin meet up with Craig Cameron to discuss his career and his work exploring RNA-dependent RNA synthesis and single cell virology. Hosts: Vincent Racaniello, Brianne Barker, and Calvin Yeager Guest: Craig Cameron Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Cameron laboratory Curioscity podcast Ribavirin is an RNA virus mutagen (Nat Med) RNA virus error catastrophe: test using ribavirin (PNAS) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

A trio TWiVers reports on influenza in Australia, how a host protein impacts bird to human movement of influenza virus, and marine DNA viral diversity in the oceans from pole to pole. Hosts: Vincent Racaniello, Kathy Spindler, and Alan Dove Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Australian Influenza Surveillance Real-time tracking of influenza H3N2 ANP32B, or not to be (eLife) ANP32B on TWiV 377 Species specific differences in ANP32B for influenza virus replication (eLife) Marine viral DNA diversity pole to pole (Cell) Letters read on TWiV 554 Timestamps by Jolene. Thanks! Weekly Science Picks Alan - Stephanie Langel's Discovery Talk Kathy- Map of languages in the USA Vincent- Grad student mental health with Susanna Harris video and on TWiM 199 Listener Picks Fernando - Pushing back on hyped-up science Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent speaks with David Oshinsky, the Pulitzer Prize-winning author of Polio: An American Story, about the history of poliovirus vaccines. Host: Vincent Racaniello Guest: David Oshinsky Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Team TWiV reveals DNA polymerases that do not require a primer, and packaging of hepatitis delta virus by the envelope glycoproteins of diverse viruses. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Brianne Barker Guest: Kiki Warren Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Bacteriophage DNA polymerase is primer-independent (PNAS) Primer-independent DNA polymerase from mobile elements (Cell Rep) Non-HBV helper viruses for HDV (Nat Comm) Waning of measles vaccine immunity (one, two, three) Letters read on TWiV 552 Timestamps by Jolene. Thanks! Weekly Science Picks Alan - Seek Rich - Want to See My Genes? Get a Warrant and NIH director will no longer speak on all-male science panels Brianne- Unique scientists Dickson- Vaccines - Calling the Shots Vincent- NY eliminates religious vaccine exemptions and Jessica Biel reveals that she does not understand vaccines Kiki- Converting type A to type O blood Listener Picks Justin - An Apple a Day Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

At Retroviruses 2019 in Cold Spring Harbor, Vincent speaks with virologist Bryan Cullen about his work and his career, together with former associates Ann Skalka, Paul Bieniasz, and Michael Malim. Host: Vincent Racaniello Guests: Bryan Cullen, Paul Bieniasz, Michael Malim, and Ann Skalka Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

TWiV explains the use of a neuronal cell line to study herpes simplex virus latency and reactivation, and a strategy for creating vaccines that induce antibodies against specific epitopes. Hosts: Vincent Racaniello, Dickson Despommier,Alan Dove, Rich Condit, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Neuronal cell line supports herpes simplex virus latency (J Virol) Protect, modify, deprotect (PNAS) Timestamps by Jolene. Thanks! Weekly Science Picks Alan - Quabbin Reservoir Rich - Katherine Swan Ginsburg Humanism in Medicine Program; The Arnold P. Gold Foundation Brianne- UPitt Measles Simulator Dickson- James Webb Space Telescope Vincent- Nobel Prizes and Life Sciences by Erling Norrby Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Kartik and Rohit join the TWiV team to present their identification of protocadherin-1 as a cell receptor for New World hantaviruses. Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler Guests: Kartik Chandran and Rohit Jangra Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Einstein goes viral (TWiV 314) Michael Rossmann, 88 (virology blog) Protocadherin-1 essential for New World hantavirus entry (Nature) Image credit Timestamps by Jolene. Thanks! This episode is sponsored by the 2019 Chem/Bio Defense Science and Technology Conference. Are you working on innovative research that can shape the future of chemical or biological defense? Submit your abstract and present your work to more than 1,500 leaders from government, academia and industry. Visit www.cbdstconference.comfor more details. Weekly Science Picks Alan - Temi and Trint Rich - Using Jupiter's moons to determine where on Earth you are Kathy- Best architecture in every state Vincent - Cyberwire Podcast: The Ghost and the Mole Kartik- Slack Rohit- Antibody responses to viral infections Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent travels to the European Congress of Virology in Rotterdam and with local co-host Marion Koopmans speak with Martin Beer, Stephan Gunther, and Vera Ross about their careers and their work on Lassa virus, Borna virus, and insect viruses. Hosts: Vincent Racanielloand Marion Koopmans Guests: Martin Beer, Stephan Gunther, and Vera Ros Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode ECV2019 Partnerships not parachutes (TWiV 413) Fatal Borna disease virus infection in transplant recipients (NEJM) Sequencing of 2018 Lassa virus outbreak (Science) Zombie caterpillar behavior (Parasitol) Timestamps by Jolene. Thanks! This episode is sponsored by the 2019 Chem/Bio Defense Science and Technology Conference. Are you working on innovative research that can shape the future of chemical or biological defense? Submit your abstract and present your work to more than 1,500 leaders from government, academia and industry. Visit www.cbdstconference.com for more details. Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent travels to the University at Albany to speak with Cara, Rachel, and Alex about their careers and their work on stress granules, epitranscriptomics, and arboviruses. Host: Vincent Racaniello Guests: Cara Pager, Rachel Netzband, and Alex Ciota Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Five postdocs in North America (TWiV 194) Zika virus subverts stress granules (J Virol) DDX68 modulates miR-122 interaction with HCV RNA (Virol) (+) RNA virus epitranscriptome (Nucl Acids Res) Adaptation of Rabensburg virus to vertebrate hosts (Virol) Effects of Zika virus strain and Aedes species on vector competence (Emerg Inf Dis) Inter- and intra-host genetic variation of West Nile virus and fine-scale evolutionary pressures (Infect Genet Evol) Timestamps by Jolene. Thanks! This episode is sponsored by the 2019 Chem/Bio Defense Science and Technology Conference. Are you working on innovative research that can shape the future of chemical or biological defense? Submit your abstract and present your work to more than 1,500 leaders from government, academia and industry. Visit www.cbdstconference.comfor more details. Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The un-encapsidated TWiV Humans discuss finding hepatitis D virus-related sequences in birds and snakes, and fatal swine acute diarrhoea syndrome caused by a coronavirus of bat origin. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode European Virus Archive ASV early bird registrationends 15 May FDA approves Dengvaxia Divergent hepatitis D-like agentin birds(Viruses) Novel deltavirus in snakes(mBio) SADS-coronavirusin piglets (Nature) Hosts and sources of endemic human coronaviruses(Adv Virus Res) Image credit Letters readon TWiV 546 Timestamps by Jolene. Thanks! This episode is sponsored by the 2019 Chem/Bio Defense Science and Technology Conference. Are you working on innovative research that can shape the future of chemical or biological defense? Submit your abstract and present your work to more than 1,500 leaders from government, academia and industry. Visit www.cbdstconference.com for more details. Weekly Science Picks Alan - Plots of Data visualization tool Rich - How to control the brain; Meet UF Physician Kelly Foote, MD Dickson- Oceanix Kathy- Iceproofing large structures Vincent - Neanderthal Man: In Search of Lost Genomes by Svante Paabo Listener Pick Johyne- NYTimes Mag Lab (sorry, no link) Sheena - History of Vaccines by NPR Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Jens Kuhn returns to TWiV to explain Select Agents, Priority Pathogens, Australia List Pathogens, Risk Group Agents, biosafety, biosecurity, and biosurety. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Brianne Barker Guest: Jens Kuhn Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Bioweapon (TWiV 508) Bioterrorism and Biocrimes by W. Seth Carus Select Agents (selectagents.gov) BMBL Manual 5th Edition (pdf) Threading the NEIDL video (YouTube) Timestamps by Jolene. Thanks! Weekly Science Picks Jens - Forvo pronunciation guide Brianne -How long do vaccines last? Alan- How much hotter is your home town than when you were born? Dickson- 50 Stunning Photos of Earth Vincent - Madness and Memory by Stanley Prusiner Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV team reveals the repertoire of anti-viral antibodies in newborn humans, and a complement protein that binds the adenovirus capsid and prevents release of the viral DNA. Hosts: Vincent Racaniello, Rich Condit, Kathy Spindler, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Maternal anti-viral antibodies in newborns (Nat Med) Complement protein blocks adenovirus uncoating (Cell Host Micr) Antibodies neutralize virus inside cells (virology blog) Image credit Letters read on TWiV 544 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - Doctors use HIV in gene therapy to fix 'bubble boy' disease Rich - I Want What My Male Colleague Has Kathy- Preventing airborne disease (video and primary article) Vincent - The Mueller Report Listener Pick Steve- Vaccine Mythbusting Cyprian- Ten simple rules towards healthier research labs Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiV team discusses Medusavirus, isolated from a hot spring in Japan, and induction of neurodegeneration by recurrent herpes simplex virus 1 infection of mice. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, Kathy Spindler, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Medusavirus (J Virol) Recurrent herpes simplex virus-1 and Alzheimer's (PLoS Path) Image credit Letters read on TWiV 543 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - How to Take a Picture of a Black Hole Alan- Jupiter Marbleimage Rich - NASA Commercial Crew Program; How SpaceX and Boeing will get Astronauts to the ISS; Everyday Astronaut Dickson- Sky-High Stunners Kathy- Arts at Michigan photo winnersand UM Microscopy Core image contest winners Vincent - NYC declares emergency over measles outbreak, mandates vaccinations and EMDataResource Listener Pick Martin- Sydney Brenner passes Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

Vincent travels to Microbiotix, Inc, a biopharmaceutical company in Worcester, MA to speak with four members of the company about their discovery and development of small molecule drugs that target serious infectious diseases. Host: Vincent Racaniello Guests: Terry Bowlin, Zach Aron, Grace Lui, and Islam Hussein Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Microbiotix, Inc. MBX-400 Microbiotix pipeline Microbiotix publications Timestamps by Jolene. Thanks! Listener Pick Islam- How to successfully collaborate with industry Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiVers present mitoviruses, which infect mitochondria, and how quasi-enveloped hepatitis A virus gets naked again. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Evidence for plant mitoviruses (Virology) Uncoating of quasi-enveloped HAV (eLife) Image credit Letters read on TWiV 541 Timestamps by Jolene. Thanks! Weekly Science Picks Alan- Where and when to see cherry blossoms Dickson- Nebraska floods Kathy- Peeps fungal infection experiment Vincent - Emergency declared in NY over measles, unvaccinated barred from public spaces Listener Pick Sam- STLR conversations Josh- Tree of Life Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]

The TWiVstars reveal the diversity of herpes simplex virus type 2 in a neonatal population, and parallel adaptation of rabbits in three countries to myxoma virus. Hosts: Vincent Racaniello, Dickson Despommier, Rich Condit, Alan Dove, and Kathy Spindler Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Diversity of HSV2 in neonates (mSphere) Adaptation of rabbits to myxoma virus (Science) Principal component analysis (YouTube) Image credit Letters read on TWiV 540 Timestamps by Jolene. Thanks! Weekly Science Picks Alan- The Quarters Rich- Brian May - New Horizons (Ultima Thule Mix) [Official Music Video] Dickson- How rats became an inescapable part of city living Kathy- Principal Component Analysis (short longer) and Educational Material about Influenza Viruses Vincent - Why "chickenpox parties" are a terrible idea—in case it's not obvious Intro music is by Ronald Jenkees. Send your virology questions and comments to [email protected]