Let's Talk Micro

In this episode Luis continues going over Corynebacterium. In the last episode he mentioned some media that is used to isolate Corynebacterium diphtheriae. Have you heard of cystine tellurite agar? How about Tinsdale agar? What type of testing is there for the diphtheria toxin?What test systems can identify C. diphtheriae? Tune in to learn more about this organism and how this media works. Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

Back to the gram-positive rods! This week Luis goes back to gram-positive rods: Corynebacterium this time. The episode starts with an overview: gram stain, biochemicals, morphology, and species. What do you know about Corynebacterium diphtheriae? Does it always cause diphtheria? What is a pseudomembrane? Tune in and download this episode to learn about this genus that has some species commonly seen in the laboratory. Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

What is antimicrobial stewardship ? What is a hospital antibiogram? In this episode Dr. Timothy Gauthier joins the podcast and talks to Luis about antimicrobial stewardship. Dr. Gauthier is a pharmacist and creator of the IDstewardship.com and Learnantibiotics.com websites. He is also the author of a book titled Learn Antibiotics. Luis asks him about what antibiotics are used to treat certain organisms. Don't miss this great episode. Timothy Gauthier's information: X: @IDstwerdship Websites: IDstewardship.com Learnantibiotics.com Book available on Amazon: https://a.co/d/9ikuZ10 Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

What is the Doctor in Public Health (DrPH) degree? What kind of work does it prepare you to do? What concentrations does this degree has? In this episode Luis talks to Dr. Jill Roberts, an associate professor at the University of South Florida (USF). Dr. Roberts is the director of the DrPH with a concentration in Public Health and Clinical Laboratory Science and Practice degree program at USF. What are the prerequisites for this degree? What are the career options? Are you able to pursue this degree if you have a background in Clinical Laboratory ? Tune in to find out. Link to USF's DrPH in Public Health and Clinical Laboratory Science and practice: https://health.usf.edu/publichealth/apply/doctoral/drph-phclsp Dr. Robert's contact info: [email protected] Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

Microbiology in high school ? Must people take biology in high school, but microbiology is not typically an option. Those of you that are microbiology lovers, would you have taken it had there been an option? Well, check out this episode where Meredith Townsend, a high school teacher joins the podcast to talk to Luis about teaching microbiology to her students. She talks about the curriculum, grading, how does it help the students, and more. Want to reach out to Meredith Townsend? See below: [email protected] --- contact me and we can get you in the Google Group! https://mertownsend34.wixsite.com/mrstownsendteaches --- here is my teaching blog! https://a.co/d/4Q3X3VK --- ABC's of Microbiology: A coloring book for future science nerds @Mer_Townsend - X username mrs_townsend_teaches --- Instagram Want to support the podcast? Here's how: Buy me a Ko-fi : https://ko-fi.com/letstalkmicro Venmo: https://venmo.com/u/letstalkmicro

In this episode Luis continues going over Listeria: testing and media. First he goes over two test systems that can detect Listeria in blood, and then he goes over media. Have you heard of Oxford or Palcalm agar? How about chromogenic agar for Listeria? Tune in to continue learning about this critical organism. Questions? Feedback? Email those to [email protected]

Luis is talking micro in this episode. We continue our journey over gram-positive rods. This time is about Listeria. What are the virulence factors of Listeria? What is its morphology? Biochemicals? Tune in to learn about this critical organism, its resemblance to another pathogen, ways of identifying it, atypical presentations, and more. Questions? Feedback? Send those to [email protected]

You requested it and it is here! Dr. Kristen Smith joins the podcast to talk about the American Board of Medical Microbiology (ABMM) certification. What is the ABMM certification? How does it help you professionally ? What are the requirements? CPEP fellowship? Tune in to listen to Dr. Smith answer these questions and more. Link to American Society for Microbiology ABMM information: https://asm.org/certifications/american-board-of-medical-microbiology-abmm Link to ClinMicroNow: https://www.clinmicronow.org Questions? Feedback? Email [email protected]

Are you familiar with the Center for Disease Control and Prevention (CDC) Antimicrobial Laboratory Network( AR Lab Network)? How about the ThermoFisher Sensititre? In this episode, Amelia Bhatnagar from the CDC joins the podcast to talk about a study that was performed to evaluate the Sensititre GN7F panel. She discusses the study and explains to the listeners what the AR Lab Network is. Tune in to learn more about this study, the AR Lab network, and the importance of evaluating the performance of Antimicrobial Susceptibility Testing (AST) systems. Link to study: https://journals.asm.org/doi/10.1128/jcm.00799-23 Link to AR Lab Network: https://www.cdc.gov/drugresistance/ar-lab-networks/domestic.html "HAI" Activity within the AR Lab Network: https://www.cdc.gov/hai/hai-ar-programs/activities/AR-lab-network.html The AR and Patient Safety Portal: https://arpsp.cdc.gov/profile/antibiotic-resistance?tab=ar-lab-network Questions? Feedback? Send those to [email protected]

The next episode of the AMR series is here! In this episode Luis and Andrea are joined by Dr. Patrick McDaneld to discuss aminoglycosides. We go over mechanism of action, what drugs are part of this class, what organisms does it have activity against, and more. We invite you to check the CLSI M100 and get familiarized with it. Questions? Suggestions? Email those at [email protected]

The field of Microbiology continues to change. Organisms were primarily identified in the clinical laboratory by biochemical testing. Then MALDI-TOF came to the lab and was incorporated to the workflow. Now labs are bringing next-generation sequencing (NGS). What is NGS? How does it work? Have you heard of Nanopore technologies? In this episode Dr. Jose Alexander and Daniel Navas from Advent Health Orlando, join the podcast to talk about NGS. They brought it to their laboratory and discuss the whole process, including the validation, challenges and more. Have any feedback or suggestions? Send those to [email protected]

Have you heard about Variovorax durovernensis? The field of Microbiology is one that continues to change, sometimes at a fast pace. With MALDI-TOF and whole genome sequencing making rapid advances, we now learn more of existing organisms and discover novel ones. Tune in to learn about how this novel species was discovered, including the testing performed and the reason behind its name. Link to article: https://www.sciencedirect.com/science/article/pii/S2590170223001152 Questions or feedback? Send an email to [email protected]

Are you familiar with the Manual of Clinical Microbiology (MCM)? If you work in a clinical microbiology laboratory you might be familiar with it. Last year the 13th edition was released. What content is in the MCM? Where is the information obtained from? What is new in this edition? Is it available online? Dr. Karen Carroll, one of the editors-in chief joins the podcast to discuss this important resource. Link to ClinMicroNow : https://www.clinmicronow.org Feedback? Suggestions? Please email those to [email protected]

It is 2024. Is your laboratory using current breakpoints? Are you familiar with the requirements? This episode features a collaboration with Breakpoints: The SIDP Podcast. In it I join the podcast along with two other guests to talk about those requirements among other things. Check out the Breakpoints podcast: https://sidp.pinecast.co More information on breakpoints toolkit: https://clsi.org/meetings/ast/breakpoints-in-use-toolkit/

Originally published as episodes 36 and 37, in this episode I go over Streptococcus agalactiae. I discuss morphology, biochemicals, and testing among other things. What is the Lancefield group of this organism? What is the significance of this organism in neonates? Any organisms that morphologically resemble it? Tune in to find out.

Originally released as episode 53, in this episode I go over Enterococcus species. What are the most clinically significant species of Enterococcus? Morphology? Biochemicals? Tune in to find out more about these organisms, and why are they so significant in healthcare settings.

Originally released as episode 58, in this episode I continue going over S. pneumoniae. This time I go over a popular antigen test. I also discuss molecular testing, including limitations and the importance of correlating the gram stain with the result. Also, have you heard of Streptococcus pseudopneumoniae?

The AMR sub-series is back! Dr. Minkey Wungwattana joins Luis and Andrea in the podcast to talk about fluoroquinolones. What is the mechanism of action of this class of antimicrobials? What organisms do they have activity against? What about breakpoints for Salmonella? Tune in to find out. Link to breakpoints episode that Andrea mentioned: https://rss.com/podcasts/letstalkmicro/475955/

This episode was originally published as episode 57. In it I go over Streptococcus pneumoniae: gram stain, morphology, diseases, and more. What is optochin? What is the bile solubility test? Tune in to find out.

This episode features a conversation with Lisa Parlich, an Infection Preventionist, with a background in Medical Laboratory Sciences. Lisa joins the podcast to talk about an order for urinalysis that was implemented in her hospital. This order is the Urinalysis with Hold order panel. Those of you that work in the laboratory might be familiar with the Urinalysis with reflex to culture order, where if an urinalysis meets the positive criteria for a culture, an order for an urine culture is reflexed, How does this new order differ from traditional orders? How does it help the patients ? How does it work? Tune in to find out.

In this episode, Dr. Pei Zhou from the Duke University School of Medicine joins the podcast to talk about LPC-233. This is a compound that shows bactericidal activity against some gram-negative rods. LPC-233 is an LpxC inhibitor. What is LpxC? How does LPC-233 works? What are some challenges of developing LpxC inhibitors? Tune in to find out. Link to article: https://doi.org/10.1126/scitranslmed.adf5668 Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

Originally released in 2021, in this episode I go over anaerobes. What are some examples of anaerobes? Why can't they grow aerobically ? What is a facultative anaerobe? Tune in to learn more.

In this episode I continue talking about MALDI-TOF MS. This time I go over the advantages and disadvantages. How has MALDI-TOF helped Clinical Microbiology? How does it compare to traditional identification methods and the workflow? What about disadvantages? Tune in to find out.

This episode features a collaboration with Jame and Callum from the ID:IOTS podcast. They are two infectious disease physicians that just like Let's Talk Micro, their goal is to share information and help educate the audience. We join forces to talk about the laboratory. We start by going over what a Medical laboratory Scientist does ( I talk about what I do). We also go over questions like what do we expect to see in a laboratory requisition. Do we add media based on what the source or the requisition indicates? How can we improve communication between the laboratory and providers? Tune in to find out. ID:IOTS podcast page: https://idiotspodcasting.buzzsprout.com Please download and review episodes

Luego de un episodio introductorio , Luis y Maridaliz regresan nuevamente con un episodio para la serie en español . En este episodio discuten medios de cultivo. Qué son los medios de cultivo? Cómo estos medios ayudan a los microorganismos? Cuáles son selectivos? Cuáles son diferenciales? Sintonizen para aprender sobre estos medios, las condiciones que los microorganismos necesitan para crecer, y más.

Following the webinar on MALDI-TOF MS, we think that now it is a good time to go over MALDI-TOF MS. This topic was discussed two years ago, but since there are new listeners, now is a good time to go over it again. Are you familiar with MALDI-TOF? How does it work? How does it help Clinical Microbiology? Tune in to find out.

This episode features a conversation with Dr. Caterina Miraglia, a professor from the University of Massachusetts Dartmouth. Dr. Miraglia published a review article about Marburg viruses in 2019, in order to educate fellow Medical Laboratory Scientists about these type of viruses. What is Marburg virus? What is Marburg virus disease? Is there any treatment or prevention? Tune in to find out.

Let's Talk Micro fue creado con el objetivo de compartir información y aprender, enseñando todo de la manera mas simple posible. Siendo fiel a esa misión , hoy comienza una serie dentro del podcast para la población que habla español . Luis Plaza, el creador y animador del podcast, se une a la Dra. Maridaliz Rodriguez para hablar de microbiología . En este episodio Luis y Maridaliz hablan de su profesión y del objetivo de esta serie.

This is a short episode to update listeners about an upcoming sub-series in Spanish. Staying true to Let's Talk Micro's goal of sharing information and learning, this sub-series is meant to do that for the Spanish speaking audience. Episodes will be released about every five weeks on a different day as regular episodes.

This episode is the one that was recorded live from ID Week 2023 in Boston. In it I talk about my favorite posters, talks, and also about my experience as a first time attendee. This episode also features a short talk with Dr. Timothy Gauthier, a Pharmacist that spends time teaching about antibiotics via a blog and publications.

This is the second part of the interview with Dr. Joel Mortensen and his experience at a hospital in Guinea. In this episode he continues talking about training laboratory personnel and teaching essential things like quality control. He also talks about issues like supplies and electricity and future plans. Are you trained in hematology and chemistry and speak French? Are you interested in helping out with training? You can email the podcast and your information will be forwarded or you can reach out to Hope Ignited. Let's Talk Micro email: [email protected] to Hope Ignited: https://www.hopeignited.org

This episode features an interview with Dr. Joel Mortensen. He is the Director of the Diagnostic Infectious Diseases Testing Laboratory at Cincinnati Children's Hospital. Dr. Mortensen volunteered to train laboratory personnel to perform microbiology testing at a pediatric hospital that was being built in Guinea, West Africa. Tune in to learn about this hospital, hear about his experience, the challenges he encountered, and more. This is part one of a two part series. Link to Hope Ignited: https://www.hopeignited.org

We have heard or recent cases of malaria in the United States. What better than to do an episode about it? Dr. Kenneth Gavina, from the Indiana University School of Medicine, joins the podcast to talk about it. Tune in to learn about its life cycle, transmission, prevention, its relationship to pregnancy, and more.Link to CDC's life cycle: https://www.cdc.gov/malaria/about/biology/index.htmlLink to Future of Malaria Research Symposium: https://publichealth.jhu.edu/malaria-research-institute/2023-future-of-malaria-research-symposium

Have you worked in a veterinary laboratory? What kind of training do you need? Are you able to work in one as an MLS? Have you thought about what a culture from a veterinary specimen might look like? How do you work those up? What about antimicrobials and guidelines? Kelli Maddock and Sarah Gefroh from the North Dakota State University Veterinary Diagnostic Laboratory join the podcast to answer these questions. They are Medical Laboratory Scientists with experience in human medicine that switched to veterinary medicine.

This episode is part two of the great emerging viruses talk with Dr. Ryan Relich and Benjamin Pinsky. They talk over diagnostic tests for viruses, challenges with test development, and they share their thoughts on viruses and outbreaks. Link to the National Academies Arbovirus workshop: https://www.nationalacademies.org/our-work/mitigating-arboviral-threats-and-strengthening-public-health-preparedness-a-workshop Link to the Pan American Society for Clinical Virology: https://www.pascv.org/default.aspx

With all the information about viruses that we see coming out, what better than an episode about emerging viruses. Dr. Benjamin Pinsky and Dr. Ryan Relich, two experienced virologists, join the podcast to talk about viruses. Tune in to learn about the different type of viruses, mode of entry, diseases, and more. This is part one of a two-part series. Link to the Clinical Virology Symposium: https://asm.org/Events/Clinical-Virology-Symposium/Home

In this episode Dr. Ryan Relich and Dr. Kenneth Gavina join the podcast to talk about an article where Francisella tularensis spp. novicida was recovered from a blood culture. Are you familiar with this organism? What are the subspecies of Francisella tularensis? What are the biochemicals tested on it and their results? While most of us might never see this organism in our careers, being aware and prepared to recognize it is essential. We have to be prepared and have a plan in place. Tune in to learn more about this organism. Link to article: https://journals.asm.org/doi/pdf/10.1128/jcm.00787-22

Season 3 kicks off with the second episode of the AMR sub-series—this time focusing on beta-lactam antibiotics. Luis and Dr. Andrea Prinzi are joined by Dr. Brian Raux, a pharmacist, to discuss beta-lactam drug classes, mechanism of action, intrinsic resistance, and CLSI guidance. A must-listen for anyone interested in antimicrobial resistance. Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

Hi everyone. Just wanted to take some time to give you some previews of upcoming episodes. From viruses, to Franciscella, to the next episode of the AMR sub-series, Let's talk Micro is kicking off season 3 with some great episodes. Also don't miss the webinar that I am presenting next week on tools and resources in a Microbiology laboratory! Registration is free. Link to sign up for webinar: https://www.clinicallab.com/trends/the-transfusion-medicine-laboratory/2023-blood-microbiology-digital-forum-27034

This episode is a collaboration with the Microbe Mail podcast. Dr. Vindana Chibabhair and I go over yeast in cultures: appropriate sources, significance, and pathogenicity versus normal flora. We also talk about instruments used to identify yeast, antifungals, and more... Check out the Microbe Mail podcast: https://microbemail.captivate.fm

This episode features a conversation with Dr. Melissa Jamerson, chair of the Medical laboratory Sciences department at Virginia Commonwealth University (VCU). Dr. Jamerson talks about the degrees offered for Medical Laboratory Sciences (MLS) at her institution, which include undergraduate and graduate. A new Masters degree with a concentration in Public Health Laboratories is starting this fall. What are the requirements for this degree? How does it differ from other Masters degrees in MLS? Tune in to find out...Link to VCU page: https://mls.chp.vcu.edu/programs/masters/

This episode is the first episode in what is going to be an Antimicrobial Resistance (AMR) sub-series. Dr. Andrea Prinzi joins me in this series. Together we breakdown this complex topic. In this episode we start with an overview of the state of AMR, Dr. Prinzi provides some history, and we explain what the series is going to be about. Learning this information can be challenging for professionals, but we are going to break it down as simple as we can. Knowing and understanding this information is a crucial part of our job as Medical Laboratory Scientists. We will go over classes of antibiotics, mechanisms of action, resistance mechanisms and more. Hope you tune in.

Digital technology is part of the laboratory. We see it on instruments in Urinalysis and Hematology where images such as those of cells are produced. What about Parasitology? Can it be used for it? Dr. Bobbi Pritt, from the Mayo Clinic Laboratory , joins the podcast to talk about it. An algorithm with Artificial Intelligence has been implemented in her lab for protozoa. How did it do? What are the components? Tune in to find out.

This episode was recorded while at the American Society for Microbiology's (ASM) Microbe 2023. I went over what I liked about this conference as a first timer. I also talk more about other courses I took and my experience overall. Then I was joined by great company: Elizabeth Deyett and Jon Mitchell from the Microbe Moment, They have a podcast called the Microbe Moment with the Microbigals. They talk about the impact of microbes in life, provide historical perspectives, and more. They also attended the conference and Elizabeth actually presented a poster. Tune in to listen to this great conversation.....Link to the Microbe Moment's podcast and webpage: https://www.microbigals.com/the-microbe-moment-science-podcast

This episode was recorded while at the American Society for Microbiology's (ASM) Microbe 2023 at Houston. I go over my favorite posters, sessions and more. It was great connecting with fellow microbiologists. Great posters such as Shewanella and oysters, frogs and potential compounds, and more. Of course, as a podcaster I had to sit through an episode of Editors in conversation.

In this episode I am joined by Dr. April Bobenchik, the Medical Microbiology Director at Penn State Health. She talks about the latest updates of the CLSI's M100. CLSI (Clinical Laboratory Standards Institute) publishes many documents, the M100 being one of them, which is updated yearly. What is the M100? How does it relate to the work that we do in Clinical Microbiology? What is new this year? Tune in to find out.CLSI's webpage: https://clsi.orgM100 webinar from CLSI link: https://clsi.org/standards/products/microbiology/education/astupdate23indwr1/Any inquiries can be emailed at [email protected]

Some of you may be familiar with the popular Parasite Wonders blog. In this episode of Let's Talk Micro, Luis is joined by its creator, Dr. Bobbi Pritt, Director of the Parasitology Laboratory at Mayo Clinic. They dive into key questions: What specimens are typically tested for parasites? What testing methods are used? Is there a role for digital parasitology? Dr. Pritt shares her insights and expertise on all things parasitic. Curious to learn more? Tune in to this fascinating conversation! Sign up for the workshop.... Link to blog: https://parasitewonders.blogspot.com Link to website: http://parasitewonders.com/ Link to workshop: https://news.mayocliniclabs.com/2023-parasitology-workshop/ Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

This episode is the conclusion of Dr. Wojewoda's story about her hospital's network going down. What was the overall effect of the network going down?What happened when the system came back up? Any lessons learned and advice? Tune in to find out. Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

Those of us that work in the laboratory have experienced a downtime at some point or another. They tend to be about 4 hours long. What if the downtime went on for days? How would you perform tasks such as add-on testing or referencing cultures? What if the whole system was down? Dr. Christina Wojewoda from the University of Vermont Medical Center joins the podcast to tell her "horror" story of when the network at her hospital went down. This is part 1 of 2 episodes. Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

This episodes features a conversation with Dr. Romney Humphries and Dr. April Abbott. They join the podcast to talk about a study where Piperacillin-Tazobactam (TZP) was evaluated. Testing results from automated systems were compared to a reference method. How did the systems perform? What are the challenges? What are CLSI breakpoints and FDA breakpoints? What is the MERINO trial? Tune in to find out. Link to article: https://journals.asm.org/doi/10.1128/jcm.01617-22 Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro