PubReading [201] - The cryo-EM method microcrystal electron diffraction (MicroED) - B. Nannenga and T. Gone

In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal electron diffraction (MicroED). Since that time, data collection and analysis schemes have been fine-tuned, and structures for more than 40 different proteins, oligopeptides and organic molecules have been determined. Here we review the MicroED technique and place it in context with other structure-determination methods. We showcase example structures solved by MicroED and provide practical advice to prospective users.

doi.org/10.1038/s41592-019-0395-x - 2019