Advancements in Microbiology Diagnostics and the 16S Ribosomal RNA Gene
Interview with Robin Patel, M.D., and Justin Kreuter, M.D. This episode discusses a “universal” gene in all bacteria,16S ribosomal RNA gene. It has regions of high conservation and areas of variability. It can be targeted by PCR in such a way that any ba
Lab Medicine Rounds · Mayo Clinic Laboratories
Audio is streamed directly from the publisher (media.transistor.fm) as published in their RSS feed. Play Podcasts does not host this file. Rights-holders can request removal through the copyright & takedown page.
Show Notes
Time Stamps
00:00 Podcast Intro
00:30 Introduction of Robin Patel, M.D. the Division Chair of Clinical Microbiology at Mayo Clinic
00:42 What is the 16S Ribosomal RNA gene?
1:28 How is it detected and sequenced in clinical microbiology?
2:33 What does this mean for physicians and their patients now that this is something that can be done in the microbiology lab?
4:23 How has the testing for this gene changed?
5:58 Can you take us through an interesting case you have come across?
9:47 What are the limitations when you’re talking about this kind of testing?
11:15 Where is this headed? Are we headed towards next-gen sequencing for 16S?
11:51 So, that will be helpful when you’re talking about using it directly on a patient sample vs. a pure colony that you’re using the testing on?
12:29 What’s the turnaround time for this kind of test?
14:08 Where does this 16S Ribosomal RNA gene testing fit in the toolbox of who should be ordering this?
15:21 What is it that you wish the medical community knew about the microbiology lab?
16:34 Outro
Resources:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652441/
https://jcm.asm.org/content/55/9/2599