Tracking grape pathogens from air to vine
In this season-opening episode of Absolute Gene-ius, hosts Jordan Ruggieri and Lisa Crawford talk with Lexi Heger, PhD candidate at Michigan State University, about applying qPCR and dPCR to understand and manage devastating grapevine pathogens. From spore trapping to fungicide resistance, Lexi shares how molecular tools are helping agriculture go greener and smarter.
Absolute Gene-ius · Lexi Heger, Lisa Crawford, Jordan Ruggieri
Show Notes
What do vineyards, airborne spores, and digital PCR have in common? Turns out – quite a lot. This episode kicks off Season 4 by going green with plant pathology and molecular diagnostics.
Lexi Heger, a PhD candidate in plant pathology and molecular plant sciences at Michigan State University, joins the show to discuss her work on grapevine diseases, particularly downy mildew caused by Plasmopara viticola. Lexi explains how she developed qPCR assays to differentiate cryptic pathogen clades, redesigned multiplex assays to track multiple grape pathogens simultaneously, and explored how digital PCR can add value when sensitivity really matters, like when detecting rare alleles linked to fungicide resistance. The conversation dives into real-world challenges like sampling air, plant, and soil environments, managing PCR inhibitors, and translating academic research into actionable tools for growers. Together, Jordan, Lisa, and Lexi highlight why qPCR and dPCR aren’t competing technologies, but complementary tools that support smarter disease management in agriculture.
In Career Corner, Lexi reflects on her lifelong love of plants, the joy of gardening, and the mentors who helped cultivate her scientific curiosity. From killing plants “to save them,” to embracing plant puns and favorite pathogens, this segment is a reminder that great science often grows from genuine passion, and a little dirt under your fingernails.
Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.