Let’s make a bet – Poisson statistics of digital PCR
For this episode we host another of Thermo Fisher Scientific’s own. Dave Bauer is a PhD-educated Application Scientist specializing in qPCR and digital PCR. His knack for using analogies to explain difficult concepts helps illuminate the benefits of digital PCR and the statistical aspects of this analytical method. This is a great overview episode that also touches on specific applications such as SNP detection. We also learn about Dave’s career path, hear some valuable advice, and get a sense of how poor our intuitions can be at evaluating probabilities.
Absolute Gene-ius · Jordan Ruggieri, Cassie McCreary
Show Notes
Polymerase chain reaction (PCR) was discovered in 1983 by Kary Mullis and Michael Smith, who were jointly awarded the Nobel Prize in Chemistry in 1993. Since then, PCR has been a cornerstone method that has been a pillar of discovery and applied science. The various types of PCR are sometimes confusing, and the relative pros and cons of each method are not always clear, which is why it’s so great to have this episode's guest explain them all in a simple and clear-cut way.
Dave Bauer, PhD, is an Application Scientist at Thermo Fisher Scientific that specializes in real time PCR (qPCR) and digital PCR (dPCR). He has an educational background in physics, mathematics, and biology, but what’s more important is that Dave loves to help others learn and to break down a topic’s complexities to make it more understandable and approachable. In this episode we hear Dave explain the difference between qPCR and dPCR, the importance of Poisson statistics to dPCR, dead volume, reaction chamber volume consistency, and more. We learn how qPCR and dPCR complement each other and how they relate to sequencing methods for applications like single nucleotide polymorphism (SNP) detection.
As you’ve come to expect from Absolute Gene-ius, you also get a good sense of who Dave is and how he got to his current role. We learn about how he knew right away that academia wasn’t for him, how he ended up unexpectedly working in forensics after his PhD, and how he eventually landed in his current Application Scientist role. Dave shares some great insights and advice, including how students should care less about their degree’s name and more about what techniques they’re learning and using in their studies.
Visit the Absolute Gene-ius page to learn more about the guest, the hosts, and the Applied Biosystems QuantStudio Absolute Q Digital PCR System.
This episode includes the following sound effects from freesound.org, licensed under CC BY-ND 4.0:
- “Sax Jazz,” by alonart
- “Balloon Pop / Christmas cracker / Confetti Cannon,” by Breviceps
- “Crowd Cheering,” by SoundsExciting
Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.